Abstract
During intracytoplasmic sperm injection (ICSI) the whole sperm, including head, midpiece and tail, is injected into the middle area of the oocyte. To find out what happens to the sperm mitochondria after ICSI, we checked the first six children born after ICSI treatment for occurrence of paternal mitochondrial DNA (mtDNA). The difference between maternal and paternal mtDNA in the investigated couples in our study was confined to single-base pair substitutions and we had to rely on restriction enzyme cleavage to differentiate between the mitochondrial genomes of the parents. With this kind of assay we were able to reach a sensitivity of about 0.2% for the paternal mtDNA. However, as uneven partition between tissues of heteroplasmic mtDNA is expected to occur, it would not be unlikely that an enrichment to 0.2% would occur in a given tissue if paternal mtDNA was transmitted by the ICSI procedure. We did not detect this level in the blood in any of the six children.
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Palermo G, Joris H, Devroey P, Van Steirteghem AC: Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 1992;340:17–18
Zamboni L, Bell J, Baca MN, Mishell DR: A penetrated human ovum studied by electron microscopy. Nature 1966;210:1373–1375
Hamberger L, Sjögren A, Lundin K, Söderlund B, Nilsson L, Bergh C: Microfertilization techniques—The Swedish Experience. Reprod Fertil Dev 1995;7:263–268
Larsson NG, Holme E, Kristiansson B, Oldfors A, Tulinius M: Progressive increase of the mutated mitochondrial DNA fraction in Kearns-Sayre syndrome. Pediatr Res 1990;28:131–136
Anderson S, Bankier AT, Barell BG, deBruijn MHL, Coulson AR, Drouin J,et al.: Sequence and organization of the human mitochondrial genome. Nature 1981;290:457–465
Houshmand M, Larsson N-G, Holme E, Oldfors A, Tulinius M, Andersen O: Automatic sequencing of mitochondrial tRNA genes in patients with mitochondrial encephalomyopathy. Biochim Biophys Acta 1994;1226:49–55
Houshmand M, Larsson N-G, Oldfors A, Tulinius MH, Holme E: Fatal mitochondrial myopathy, lactic acidosis, and complex I deficiency associated with a heteroplasmic A→G mutation at position 3251 in the mitochondrial tRNALeu(UUR) gene. Hum Genet 1996;97:269–273
Giles R, Blance H, Cann H, Wallace DC: Maternal inheritance of human mitochondrial DNA. Proc Natl Acad Sci USA 1980;77:6715–6719
Gyllensten U, Wharton D, Wilson A: Maternal inheritance of mitochondrial DNA during backcrossing of two species of mice. J Hered 1985;76(5):321–324
Hayashi J-I, Tagashira Y, Yoshida M, Ajiro K, Sekiguchi T: Two distinct types of mitochondrial DNA segregation in mouse-rat hybrid cells. Exp Cell Res 1983;147:51–61
Kaneda H, Hayashi J-I, Takahama S, Taya C, Lindahl KF, Yonekawa H: Elimination of paternal mitochondrial DNA in intraspecific crosses during early mouse embryogenesis. Proc Natl Acad Sci USA 1995;92:4542–4546
Tesarik J, Sousa M, Testart J: Human oocyte activation after intracytoplasmic sperm injection. Hum Repord 1994;9:511–518
Chen X, Prosser R, Simonetti S, Sadlock J, Jagiello G, Schon EA: Rearranged mitochondrial genomed are present in human oocytes. Am J Hum 1995;57:238–247
Birky C: Relaxed cellular controls and organelle heredity. Science 1983;222:468–475
Hecht N, Liem H, Kleene K, Distel R, Ho S-M: Maternal inheritance of the mouse mitochondrial genome is not mediated by a loss or gross alteration of the paternal mitochondrial DNA or by methylation of the occyte mitochondrial DNA. Dev Biol 1984;102:452–461
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Houshmand, M., Holme, E., Hanson, C. et al. Is paternal mitochondrial DNA transferred to the offspring following intracytoplasmic sperm injection?. J Assist Reprod Genet 14, 223–227 (1997). https://doi.org/10.1007/BF02766114
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DOI: https://doi.org/10.1007/BF02766114