Abstract
Purpose: Our objective was to apply ooplasmic round spermatid nuclear injections for the treatment of nonobstructive azoospermia.
Materials: Participants were nine azoospermic men who had previously undergone diagnostic testicular biopsy. Spermatogenetic arrest was diagnosed at the round spermatid stage (n=6) or primary spermatocyte stage (n=3). A second (therapeutic) testicular biopsy was performed and round spermatid nuclei were recovered from all the participants.
Results: Forty-nine mature oocytes were successfully injected with nuclei and then cultured for 72 hr. Twenty-four embryos were transferred to nine women. No pregnancy was achieved.
Conclusions: Round spermatids can be recovered from therapeutic testicular biopsy material of men negative for round spermatids in previous routine diagnostic testicular biopsy specimens. Round spermatid nuclear injections may play a role in the treatment of nonobstructive azoospermia.
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Yamanaka, K., Sofikitis, N.V., Miyagawa, I. et al. Ooplasmic round spermatid nuclear injection procedures as an experimental treatment for nonobstructive azoospermia. J Assist Reprod Genet 14, 55–62 (1997). https://doi.org/10.1007/BF02765754
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DOI: https://doi.org/10.1007/BF02765754