Tumour visualization in a murine model by time-delayed fluorescence of sulphonated aluminium phthalocyanine
Mice bearing the MS-2 fibrosarcoma were administered 0.25, 0.5 or 1 mg kg-1 body weight (b.w.) of sulphonated aluminium phthalocyanine (AlS2Pc) (with average degree of sulphonation of 2.1), and time-gated fluorescence images were acquired up to 6 h after the injection. Different excitation wavelengths (610, 650 and 670 nm) were tested. Red light excitation and 3 ns delayed detection allow one to minimize natural fluorescence and scattered laser light, respectively. The best conditions for tumour detection are reached under either 650 or 670 nm Excitation, 2–4 h after the administration of either 0.5 or 1 mg kg-1 b.w. of AlS2Pc. In these situations, the average fluorescence contrast between tumour area and surrounding healthy tissue is > 2, providing a clear identification of the pathological region. However, tumour localization is possible even after the injection of 0.25 mg kg-1 b.w. of sensitizer. In conclusion, under low power excitation (< 100ΜW cm-2), the technique allows real time detection of an intradermal tumour with good contrast.
Key wordsFluorescence Imaging Phthalocyanine Timegated imaging Tumour detection
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