Abstract
Nothofagus leoni has a restricted distribution in Chilean forests. This work determines suitable culture conditions forin vitro multiplication and rooting through shoots obtained from seedlings. Broadleaved Tree Medium was suitable for shoot multiplication. A medium with a pulse of 0.55 µM BA in the first subculture and two subcultures on BA-free medium resulted in a multiplication rate at day 63 of × 5.7, without callus growth or shoot neoformation. Rooted shoots of good quality (number and length of roots without callus growth) were obtained with 1.23 µM IBA (91.4% of rooting). The first roots appeared at day 11, reaching a higher speed of rooting at day 15.
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Literature cited
Bennett, I.J., McComb, J.A., Tonkin, C., and McDavid, D. (1994) Alternating cytokinins in multiplication media stimulatesin vitro shoot growth and rooting ofEucalyptus globulus Labill. Ann. Bot. 74: 53–58.
Burns, B. (1993) Fire-induced dynamics ofAraucaria araucana — Nothofagus antarctica forest in the southern Andes. J. Biogeogr. 20: 669–685.
Calderón-Baltierra, X., Pérez, F., Salazar, C., and Sanhueza, V. (1994) Physiological aspects onin vitro preserving chilean native forest species:Gomortega keule andNothofagus alpina. Noticiero Biol. 2(3): 15.
Chalupa, V. (1983) Micropropagation of conifer and broadleaved forest trees. Commun. Inst. For. Cechosloveniae 13: 7–39.
Donoso Zegers, C. (1979) Genecological differentiation inNothofagus obliqua (Mirb) Oerst in Chile. For. Ecol. Manage. 2: 53–66.
Donoso Zegers, C. (1993) Bosques templados de Chile y Argentina: Variación, Estructura y Dinámica. Ecología Forestal. 484 pp (Editorial Universitaria - CONAF).
Donoso Zegers, C. and Landrum, L. (1976)Nothofagus leoni: hibridación e introgresión en poblaciones deN. obliqua yN. glauca. Facultad de Ciencias Forestales, boletín técnico no 36. Universidad de Chile. 30 pp.
Hammerschlag, F. (1982) Factors influencingin vitro multiplication and rooting of the Plum Rootstock Myrobalan (Prunus cerasifera Ehrh). J. Am. Soc. Hortic. Sci. 107(1): 44–47.
Hill, R. and Jordan, G. (1993) The evolutionary history ofNothofagus (Nothofagaceae). Aust. Syst. Bot. 6: 111–126.
Jordan, M., Velozo, J., and Sabja, A. (1996) Organogenesisin vitro ofNothofagus alpina, Fagaceae. Plant Cell Rep. 15: 795–798.
Kataeva, N., Alexandrova, I., Butenko, R., and Dragavtceva, E. (1991) Effect of applied and internal hormones on vitrification and apical necrosis of different plants culturedin vitro. Plant Cell Tissue Organ Cult. 32: 47–53.
Martínez Pastur, G. and Arena, M. (1995)In vitro propagation ofNothofagus obliqua (Fagaceae). Aust. J. Bot. 43: 601–607.
Martínez Pastur, G. and Arena, M. (1996)In vitro propagation ofNothofagus nervosa. Phyton 58: 1–7.
Martínez Pastur, G., Pinedo, L., and Fernández, C. (1997a) Germinación de semillas y sistemas de propagación clonal en bosques deNothofagus pumilio de Tierra del Fuego. Proceedings of IIo Congreso Forestal Argentino y Latinoamericano. Posadas (Argentina), 13–15 August, 141–147.
Martínez Pastur, G., Arena, M., and Caso, O. (1997b)In vitro propagation ofNothofagus antarctica from sphaeroblasts and saplings. Biocell 21(3): 231–236.
Nadel, B., Altman, A., Pleban, S., and Huttermann, A. (1991a)In vitro development of MatureFagus sylvatica L buds. I. The effect of medium and plant growth regulators on bud growth and protein profiles. J. Plant Physiol. 138: 596–601.
Nadel, B., Altman, A., Pleban, S., and Huttermann, A. (1991b)In vitro development of MatureFagus sylvatica L buds. II. Seasonal changes in the response to plant growth regulators. J. Plant Physiol. 138: 136–141.
Németh, G. (1986) Induction of rooting.In Biotechnology in agriculture and forestry. Vol. 1: Trees I. Bajaj, Y.P.S. (ed.), Springer-Verlag, New York, 49–64.
Nielsen, J.M., Hansen, J., and Brandt, K. (1995) Synergism of thidiazuron and benzyladenine in axillary shoot formation depends on sequence of application inMiscanthus × ogiformis ‘Giganteus.’ Plant Cell Tissue Organ Cult. 41: 165–170.
Van Nieuwkerkm, J.P., Zimmerman, R.H., and Fordham, I. (1986) Thidiazuron stimulation of apple shoot proliferationin vitro. HortScience 21: 516–518.
Veblen, T. and Markgraf, V. (1988) Steppe expansion in Patagonia? Q. Res. 30: 331–338.
Vieitez, A.M., Ferro, E., and Ballester, A. (1993a) Micropropagation ofFagus sylvatica L.In vitro Cell Dev. Biol. 29P: 183–188.
Vieitez, A.M., Pintos, F., San José, M.C., and Ballester, A. (1993b)In vitro shoot proliferation determined by explant orientation of juvenile and matureQuercus rubra L. Tree Physiol. 12: 107–117.
Vieitez, A.M., Concepción Sánchez, M., Amo Marco, J.B., and Ballester, A. (1994) Forced flushing of branch segments as a method for obtaining reactive explants of matureQuercus robur trees for micropropagation. Plant Cell Tissue Organ Cult. 37: 287–295.
Von Arnold, S. and Tillberg, E. (1987) The influence of cytokinin pulse treatments on adventitious bud formation on vegetative buds ofPicea abies. Plant Cell Tissue Organ Cult. 9: 253–261.
Zimmerman R. (1984) Rooting apple cultivarsin vitro: Interactions among light, temperature, phloroglucinol and auxin. Plant Cell Tissue Organ Cult. 3: 301–311.
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Martínez Pastur, G.J., Arena, M.E. In Vitro propagation of juvenileNothofagus leoni Espinosa (Fagaceae). J For Res 4, 295–298 (1999). https://doi.org/10.1007/BF02762787
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DOI: https://doi.org/10.1007/BF02762787