Journal of Forest Research

, Volume 4, Issue 4, pp 295–298 | Cite as

In Vitro propagation of juvenileNothofagus leoni Espinosa (Fagaceae)

  • Guillermo J. Martínez Pastur
  • Miriam E. Arena
Short Communication


Nothofagus leoni has a restricted distribution in Chilean forests. This work determines suitable culture conditions forin vitro multiplication and rooting through shoots obtained from seedlings. Broadleaved Tree Medium was suitable for shoot multiplication. A medium with a pulse of 0.55 µM BA in the first subculture and two subcultures on BA-free medium resulted in a multiplication rate at day 63 of × 5.7, without callus growth or shoot neoformation. Rooted shoots of good quality (number and length of roots without callus growth) were obtained with 1.23 µM IBA (91.4% of rooting). The first roots appeared at day 11, reaching a higher speed of rooting at day 15.

Key words

cytokinin pulse in vitro multiplication Nothofagus leoni rooting 


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Copyright information

© The Japanese Forest Society and Springer 1999

Authors and Affiliations

  • Guillermo J. Martínez Pastur
    • 1
  • Miriam E. Arena
    • 1
  1. 1.Centro Austral de Investigaciones CientíficasConsejo Nacional de Investigaciones Científicas y Técnicas. cc 92 (9410) UshuaiaTierra del FuegoArgentina

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