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Molecular Biotechnology

, Volume 6, Issue 1, pp 87–89 | Cite as

Simple procedure for estimating the efficiency of PCR

  • Aldar S. Bourinbaiar
  • Kenneth Ajuang-Simbiri
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Abstract

The extension efficiency of PCR-driven DNA amplification was examined by step-controlled limiting dilution method using as internal controls 8E5 lymphocytes carrying a single copy of HIV genome. The results reveal that under standard conditions recommended by the PCR kit manufacturer the exponential growth of DNA template is approximately one third of the theoretical doubling rate.

Index entries

Amplification efficiency PCR quantitation HIV 

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Copyright information

© Humana Press Inc. 1996

Authors and Affiliations

  • Aldar S. Bourinbaiar
    • 1
  • Kenneth Ajuang-Simbiri
    • 2
  1. 1.Metatron Inc.New York
  2. 2.Zanexis Inc.Malvern

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