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Molecular Biotechnology

, Volume 6, Issue 3, pp 287–310 | Cite as

Phage-display libraries of murine and human antibody Fab fragments

  • Jan Engberg
  • Peter Sejer Andersen
  • Leif Kofoed Nielsen
  • Morten Dziegiel
  • Lene K. Johansen
  • Bjarne Albrechtsen
Protocol

Abstract

We provide efficient and detailed procedures for construction, expression, and screening of comprehensive libraries of murine or human antibody Fab fragments displayed on the surface of filamentous phage. In addition, protocols for producing and using ultra-electrocompetent cells, for producing Fab phages from libraries, and for selecting antigen binders by panning are presented. The latter protocol includes a procedure for trypsin elution of bound phage.

Index Entries

Assembly PCR Fab libraries phage display phagemid vectors recombinant antibodies 

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Copyright information

© Humana Press Inc. 1996

Authors and Affiliations

  • Jan Engberg
    • 1
  • Peter Sejer Andersen
    • 1
  • Leif Kofoed Nielsen
    • 2
  • Morten Dziegiel
    • 2
  • Lene K. Johansen
    • 1
  • Bjarne Albrechtsen
    • 1
  1. 1.Department of BiologyThe Royal Danish School of PharmacyCopenhagenDenmark
  2. 2.Blodbanken KI2031, Department of Clinical ImmunologyUniversity HospitalCopenhagenDenmark

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