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Russian Journal of Bioorganic Chemistry

, Volume 26, Issue 8, pp 721–724 | Cite as

The MALDI mass spectrometry in the identification of new proteins in snake venoms

  • V. V. Kukhtina
  • C. Weise
  • A. V. Osipov
  • V. G. Starkov
  • M. I. Titov
  • S. E. Esipov
  • T. V. Ovchinnikova
  • V. I. Tsetlin
  • Yu. N. Utkin
Article

Abstract

By MALDI MS, we searched cobra venoms for new low-content polypeptides. A number of new proteins with molecular masses 7–25 kDa, characteristic of the known snake protein toxins, were identified, with the content of one of them less than 0.02%.

Key words

cobra venom MALDI mass spectrometry polypeptide toxins proteins 

Abbreviations

AChR

acetylcholine receptor

MALDI

matrix-assisted laser desorption ionization

MS

mass spectrometry

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Copyright information

© MAIK “Nauka/Interperiodica” 2000

Authors and Affiliations

  • V. V. Kukhtina
    • 1
  • C. Weise
    • 2
  • A. V. Osipov
    • 1
  • V. G. Starkov
    • 1
  • M. I. Titov
    • 1
  • S. E. Esipov
    • 1
  • T. V. Ovchinnikova
    • 1
  • V. I. Tsetlin
    • 1
  • Yu. N. Utkin
    • 1
  1. 1.Shemyakin-Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesGSP-7 MoscowRussia
  2. 2.Institute of Chemistry and BiochemistryFree University of BerlinBerlinGermany

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