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The MALDI mass spectrometry in the identification of new proteins in snake venoms

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Abstract

By MALDI MS, we searched cobra venoms for new low-content polypeptides. A number of new proteins with molecular masses 7–25 kDa, characteristic of the known snake protein toxins, were identified, with the content of one of them less than 0.02%.

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Abbreviations

AChR:

acetylcholine receptor

MALDI:

matrix-assisted laser desorption ionization

MS:

mass spectrometry

References

  1. Tsetlin, V.,Eur. J. Biochem., 1999, vol. 264, pp. 281–286.

    Article  PubMed  CAS  Google Scholar 

  2. Grant, G.A., Luetje, C.W., Summers, R., Xu, X.L.,Biochemistry, 1998, vol. 37, pp. 12 166–12 171.

    CAS  Google Scholar 

  3. Loring, R.H.,J. Toxicol.-Toxin Reviews, 1993, vol. 12, pp 105–153.

    CAS  Google Scholar 

  4. Chiappinelli, V.A., Weaver, W.R., McLane, K.E., Conti-Fine, B.M., Fiordalisi, J.J., and Grant, G.A.,Toxicon, 1996, vol. 34, pp. 1243–1256.

    Article  Google Scholar 

  5. Jerusalinsky, D., Kornisiuk, E., Alfaro, P., Quillfeldt, J., Ferreira, A., Rial, V.E., Duran, R., and Cervenansky, C.,Toxicon, 2000, vol. 38, pp. 747–761.

    Article  PubMed  CAS  Google Scholar 

  6. Liang, J.S., Carsi-Gabrenas, J., Krajewski, J.L., McCafferty, J.M., Purkerson, S.L., Santiago, M.P., Strauss, W.L, Valentine, H.H., and Potter, L.T.,Toxicon, 1996, vol. 34, pp. 1257–1267.

    Article  Google Scholar 

  7. Changeux, J.-P. and Edelstein, S.J.,Neuron, 1998, vol. 21, pp. 959–980.

    Article  PubMed  CAS  Google Scholar 

  8. Jan, L.Y. and Jan, Y.N.,Annu. Rev. Neurosci., 1997, vol. 20, pp. 91–123.

    Article  PubMed  CAS  Google Scholar 

  9. Altschul, S.F. and Gish, W.,Methods Enzymol., 1996, vol. 266, pp. 460–480.

    Article  Google Scholar 

  10. Altschul, S.F., Madden, T., Schaffer, A., Zhang J., Zhang, Z., Miller, W., and Lipman, D.J.,Nucleic Acids Res., 1997, vol. 25, pp. 3389–3402.

    Article  PubMed  CAS  Google Scholar 

  11. Joubert, F.J. and Taljaard, N.,Hoppe-Seyler’s Z. Physiol. Chem., 1980, vol. 361, pp. 425–436.

    PubMed  CAS  Google Scholar 

  12. Utkin, Yu.N., Kukhtina, V.V., Maslennikov, I.V., Eletsky, A.V., Starkov, V.G., Weise, C., Franke P., Hucho, F., and Tsetlin, V.I.,Toxicon, 2000 (in press).

  13. Tu, A.T.,Venoms: Chemistry and Molecular Biology, New York: Wiley, 1977.

    Google Scholar 

  14. Wei, A.L. and Lee, C.Y.,Toxicon, 1965, vol. 3, pp. 1–4.

    Article  PubMed  CAS  Google Scholar 

  15. Lee, C.Y., Chang, S.L., Kau, S.T., and Luh, S.-H.,J. Chromatogr., 1972, vol. 72, pp. 71–82.

    Article  PubMed  CAS  Google Scholar 

  16. Kussmann, M. and Nordhoff, E.,J. Mass-Spec., 1997, vol. 32, pp. 593–601.

    Article  CAS  Google Scholar 

  17. Dawson, R.M.C., Elliott, D.C., Elliot, W.H., and Jones, K.M.,Data for Biochemical Research, Oxford: Clarendon, 1986. Translated under the titleSpravochnik biokhimika, Moscow: Mir, 1991.

    Google Scholar 

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Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117871, GSP-7 Moscow, Russia

    V. V. Kukhtina, A. V. Osipov, V. G. Starkov, M. I. Titov, S. E. Esipov, T. V. Ovchinnikova, V. I. Tsetlin & Yu. N. Utkin

  2. Institute of Chemistry and Biochemistry, Free University of Berlin, Berlin, Germany

    C. Weise

Authors
  1. V. V. Kukhtina
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  2. C. Weise
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  3. A. V. Osipov
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  4. V. G. Starkov
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  5. M. I. Titov
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  6. S. E. Esipov
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  7. T. V. Ovchinnikova
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  8. V. I. Tsetlin
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  9. Yu. N. Utkin
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Corresponding author

Correspondence to Yu. N. Utkin.

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Kukhtina, V.V., Weise, C., Osipov, A.V. et al. The MALDI mass spectrometry in the identification of new proteins in snake venoms. Russ J Bioorg Chem 26, 721–724 (2000). https://doi.org/10.1007/BF02759624

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  • DOI: https://doi.org/10.1007/BF02759624

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