Abstract
The cobalt(II) tetracarboxyphthalocyanine-deoxyribonucleotide pd(TCTTCCCA) conjugate was synthesized. The phthalocyanineN-succinimide ester prepared from phthalocyanine using DCC was mixed in DMF with an aqueous solution of the oligonucleotide bearing a 1,3-diaminopropane linker at the 5′-phosphate. The resulting conjugate was tested in the intraduplex reaction with target 14-mer and 22-mer oligonucleotides containing conjugate-complementary sequences. In the presence of O2 and a thiol (2-mercaptoethanol or DTT), as a coupled reducer, or H2O2, sequence-specific DNA modification was observed that caused the cleavage of the target upon treatment with piperidine.
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Abbreviations
- Ptc:
-
phthalocyanine residue
- DMAP:
-
4-(dimethylamino)pyridine
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This article is dedicated to the 25th Anniversary of the journal Bioorganicheskaya Khimiya
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Koval, V.V., Chernonosov, A.A., Abramova, T.V. et al. The synthesis of a cobalt(II) tetracarboxyphthalocyanine-deoxyribooligonucleotide conjugate as a reagent for the directed DNA modification. Russ J Bioorg Chem 26, 104–110 (2000). https://doi.org/10.1007/BF02759155
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DOI: https://doi.org/10.1007/BF02759155