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Russian Journal of Bioorganic Chemistry

, Volume 26, Issue 2, pp 104–110 | Cite as

The synthesis of a cobalt(II) tetracarboxyphthalocyanine-deoxyribooligonucleotide conjugate as a reagent for the directed DNA modification

  • V. V. Koval
  • A. A. Chernonosov
  • T. V. Abramova
  • T. M. Ivanova
  • O. S. Fedorova
  • D. G. Knorre
Article

Abstract

The cobalt(II) tetracarboxyphthalocyanine-deoxyribonucleotide pd(TCTTCCCA) conjugate was synthesized. The phthalocyanineN-succinimide ester prepared from phthalocyanine using DCC was mixed in DMF with an aqueous solution of the oligonucleotide bearing a 1,3-diaminopropane linker at the 5′-phosphate. The resulting conjugate was tested in the intraduplex reaction with target 14-mer and 22-mer oligonucleotides containing conjugate-complementary sequences. In the presence of O2 and a thiol (2-mercaptoethanol or DTT), as a coupled reducer, or H2O2, sequence-specific DNA modification was observed that caused the cleavage of the target upon treatment with piperidine.

Key words

DNA modification oligonucleotide phthalocyanine 

Abbreviations

Ptc

phthalocyanine residue

DMAP

4-(dimethylamino)pyridine

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Copyright information

© MAIK “Nauka/Interperiodica” 2000

Authors and Affiliations

  • V. V. Koval
    • 1
  • A. A. Chernonosov
    • 2
  • T. V. Abramova
    • 1
  • T. M. Ivanova
    • 1
  • O. S. Fedorova
    • 1
  • D. G. Knorre
    • 1
  1. 1.Novosibirsk Institute of Bioorganic Chemistry, Siberian DivisionRussian Academy of SciencesNovosibirskRussia
  2. 2.Novosibirsk State UniversityNovosibirskRussia

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