Instruments and Experimental Techniques

, Volume 43, Issue 3, pp 430–433 | Cite as

High-vacuum cryomicrotome with a single bellows input of the sample motion and cooling

  • D. A. Moshkov
  • A. A. Khokhlov
  • E. A. Kirina
  • B. D. Bologov
Laboratory Techniques


A cryomicrotome based on a heated inlet of liquid nitrogen and complex (an 8-mm-amplitude reciprocating and circular rotary about the vertical axis) motion allows one to conduct all freeze-fracturing operations under visual control (the temperatures of the object and knife are −115°C and −180°C, respectively) and to obtain high-quality replicas of the studied biological sample in the operation chamber with a volume below 1.11 under an oil-free vacuum no worse than 10−5 Pa, created by a turbo-molecular pump.


Flange Evacuation System Object Holder Rotary Valve Preliminary Evacuation 
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  1. 1.
    Komissarchik, Ya.Yu. and Mironov, A.A.,Elektronnaya mikroskopiya (Electron Microscopy), Leningrad: Nauka, 1990, p. 143.Google Scholar
  2. 2.
    Pipko, A.I., Pliskovskii, V.Ya., and Penchko, E.A.,Konstruirovanie i raschet vakuumnykh sistem (Design and Calculation of Vacuum Systems), Moscow: Energiya, 1979, p. 504.Google Scholar
  3. 3.
    Gross, H.,Acta Histochemica, 1981, vol. 23, p. 21.Google Scholar
  4. 4.
    Mednikov, V.I.,Vvody dvizheniya v vakuum (Conpling Motion into Vacuum), Moscow: Mashinostroenie, 1972, p. 184.Google Scholar
  5. 5.
    Bologov, B.D., Moshkov, D.A., and Khokhlov, A.A.,Prib. Tekh. Eksp., 1998, no. 3, p. 128.Google Scholar

Copyright information

© MAIK “Nauka/Interperiodica” 2000

Authors and Affiliations

  • D. A. Moshkov
    • 1
  • A. A. Khokhlov
    • 2
  • E. A. Kirina
    • 3
  • B. D. Bologov
    • 1
  1. 1.Institute of Theoretical and Experimental BiophysicsRussian Academy of SciencePushchino, Moscow oblastRussia
  2. 2.Institute of Cell BiophysicsRussian Academy of SciencePushchino, Moscow oblastRussia
  3. 3.Pushchino Branch of Moscow State Academy of Instrumentation and InformaticsPushchino, Moscow oblastRussia

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