High-vacuum cryomicrotome with a single bellows input of the sample motion and cooling
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A cryomicrotome based on a heated inlet of liquid nitrogen and complex (an 8-mm-amplitude reciprocating and circular rotary about the vertical axis) motion allows one to conduct all freeze-fracturing operations under visual control (the temperatures of the object and knife are −115°C and −180°C, respectively) and to obtain high-quality replicas of the studied biological sample in the operation chamber with a volume below 1.11 under an oil-free vacuum no worse than 10−5 Pa, created by a turbo-molecular pump.
KeywordsFlange Evacuation System Object Holder Rotary Valve Preliminary Evacuation
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