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Russian Journal of Developmental Biology

, Volume 31, Issue 5, pp 309–313 | Cite as

Analysis of adhesion proteins from neural eye tissues of eight-day-old chick embryos

  • M. S. Krasnov
  • E. N. Grigoryan
  • V. P. Yamskova
  • A. N. Danilenko
  • I. A. Yamskov
Original Articles
  • 21 Downloads

Abstract

Two groups of proteins were isolated from the retina and pigment epithelium of eight-day-old chick embryos. Experiments with suspension cultures of retinal cells demonstrated that only the retinal extracts and the fraction of its acidic proteins can stimulate cell aggregation in vitro. Analysis by the method of high-performance liquid chromatography showed that fractions of acidic and basic retinal proteins, which markedly differ in their electric charge and biological activity, have similar composition. To study the effect of these proteins on the morphological and functional state of pigment epitheliumin vitro, a new experimental model is proposed, with the posterior segment of the newt (Pleurodeles waltl) eye used as a test tissue. The fraction of basic proteins isolated from the chick embryonic pigment epithelium stabilized cell differentiation in the newt pigment epithelium. The analyzed proteins proved to be biologically active at extremely low doses, corresponding to 10−12 M solutions.

Key words

adhesion proteins retinal pigment epithelium differentiation newts 

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Copyright information

© MAIK “Nauka/Interperiodica” 2000

Authors and Affiliations

  • M. S. Krasnov
    • 1
  • E. N. Grigoryan
    • 1
  • V. P. Yamskova
    • 1
  • A. N. Danilenko
    • 2
  • I. A. Yamskov
    • 2
  1. 1.Kol’tsov Institute of Developmental BiologyRussian Academy of SciencesMoscowRussia
  2. 2.Nesmeyanov Institute of Organoelement CompoundsRussian Academy of SciencesMoscowRussia

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