Advertisement

Russian Journal of Bioorganic Chemistry

, Volume 26, Issue 1, pp 54–59 | Cite as

The isolation, preliminary structural studies, and physiological activity of water-soluble polysaccharides from the squeezed berries of the snowball treeViburnum opulus

  • R. G. Ovodova
  • V. V. Golovchenko
  • S. V. Popov
  • A. S. Shashkov
  • Yu. S. Ovodov
Article

Abstract

Water-soluble polysaccharide fractions VO1–VO4 were isolated from the squeezed berries of the snowball tree (Viburnum opulus) by successive extraction with water at various temperatures and pH and with aqueous solutions of ammonium oxalate. These fractions were purified by ion-exchange chromatography on DEAE cellulose, and the homogeneity of the purified polysaccharides was determined by gel filtration on Sephacryl S-500. Acidic polysaccharides close to pectins in their sugar composition were found in all the extracts (fractions VO1-1, VO2-1, VO3-2, and VO4-2). Residues of galacturonic acid, galactose, arabinose, and (to a lesser extent) rhamnose are their main constituents. Neutral polysaccharides composed mainly of galactose and mannose residues were additionally found in fractions extracted with acidified water (pH 4.0) and with aqueous ammonium oxalate solutions. Partial acidic hydrolysis and digestion with pectinase of acidic polysaccharides indicated that their carbohydrate backbone consists of α-1,4-linked residues ofD-galacturonic acid. NMR spectra of acidic polysaccharides (fractions VO3-2 and VO3-3) confirmed this and demonstrated that their side oligosaccharide chains are composed of β-1,4-linked galactopyranose residues and of terminal and 2,5- and 3,5-substituted residues of α-arabinofuranose at a Gal : Ara ratio of 3 : 1. Some polysaccharides fromV. opulus were found to possess an immunostimulating activity: they enhance phagocytosis, in particular, the phagocytic index and the secretion of lysosomal enzymes with peritoneal macrophages. Calcium ions were found to be necessary for the appearance of the stimulating effect of acidic polysaccharides fromV. opulus.

Key words

plant polysaccharides Viburnum opulus mucilages of seeds pectins arabinogalactans NMR spectroscopy of polysaccharides immunomodulators 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Ovodov, Yu.S.,Bioorg. Khim., 1998, vol. 24, pp. 483–501.PubMedGoogle Scholar
  2. 2.
    Stepanova, T.A., Kaminskaya, A.V., Derkach, A.I., and Komissarenko, N.F.,Rast. Resursy, 1998, vol. 34, pp. 21–34.Google Scholar
  3. 3.
    Ovodova, R.G., Golovchenko, V.V., and Popov, S.V.,Khimiya Rast. Syr’ya, 1999, no. 1, pp. 53–57.Google Scholar
  4. 4.
    Stephen, A.M.,The Polysaccharides, Aspinall, G.O., Ed., New York: Academic, 1983, vol. 2, pp. 98–195.Google Scholar
  5. 5.
    Jansson, P.-E., Kenne L., and Widmalm, G.,Carbohydr. Res., 1989, vol. 188, pp. 169–191.PubMedCrossRefGoogle Scholar
  6. 6.
    Usov, A.I., Bilan, M.I., and Klochkova, N.G.,Bot. Marina, 1995, vol. 38, pp. 43–51.CrossRefGoogle Scholar
  7. 7.
    Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J.,J. Biol. Chem., 1951, vol. 193, pp. 265–275.PubMedGoogle Scholar
  8. 8.
    Wood, P.J. and Siddiqui, I.R.,Anal. Biochem., 1971, vol. 39, pp. 418–423.PubMedCrossRefGoogle Scholar
  9. 9.
    York, W.S., Darvill, A.G., McNeil, M., Stevenson, T.T., and Albersheim, P.,Methods Enzymol., 1985, vol. 118, pp. 3–40.CrossRefGoogle Scholar
  10. 10.
    Perepelov, A.V., Babicka, D., Shashkov, A.S., Arbatsky, N.P., Senchenkova, S.N., Rozalski, A., and Knirel, Y.A.,Carbohydr. Res., 1999, vol. 318, pp. 186–192.PubMedCrossRefGoogle Scholar
  11. 11.
    Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., and Smith, F.,Anal. Chem., 1956, vol. 28, pp. 350–356.CrossRefGoogle Scholar
  12. 12.
    Hunt, C.,Lymphocytes: A Practical Approach, Washington: IRL, 1989. Translated under the titleLimfotsity. Metody, Moscow: Mir, 1990.Google Scholar
  13. 13.
    Khaitov, R.M., Pinegin, B.V., and Istamov, Kh.I.,Ekologicheskaya immunologiya (Ecological Immunology), Moscow: Vses. Inst. Rybov. Okeanogr., 1995.Google Scholar

Copyright information

© MAIK “Nauka/Interperiodica” 2000

Authors and Affiliations

  • R. G. Ovodova
    • 1
  • V. V. Golovchenko
    • 1
  • S. V. Popov
    • 1
  • A. S. Shashkov
    • 2
  • Yu. S. Ovodov
  1. 1.Institute of Physiology, Komi Research Center, Urals BranchRussian Academy of SciencesSyktyvkarRussia
  2. 2.Zelinskii Institute of Organic ChemistryRussian Academy of SciencesMoscowRussia

Personalised recommendations