Russian Journal of Developmental Biology

, Volume 31, Issue 3, pp 166–170 | Cite as

Nitrosomethylurea disturbs differentiation of mouse embryonic lungs in organ cultures

  • N. V. Popova
  • L. Rossi
Experimental Embryology


We have studied the effect of nitrosomethylurea (NMU) on the differentiation of early rudiments of mouse embryonic lungs (12th day of embryogenesis) explanted into an organ culture. We have demonstrated that nontoxic doses of NMU are capable of accelerating normal lung differentiation both at the early (increase in the number of epithelial buds) and at the late (increase in the number of explants with regions of well-developed alveoli) stages of cultivation. However, NMU induces disturbances of differentiation, which appear as polycystic structures and hyperplastic nodules generally absent in the control.

Key words

growth factors embryonic lungs organ cultures 


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  1. Bost, F., McKay, R., Dean, N.,et al., The JUN Kinase/Stress-Activated Protein Kinase Pathway Is Required for Epidermal Growth Factor Stimulation of Growth of Human A549 Lung Carcinoma Cells,J. Biol. Chem., 1997, vol. 272, no. 52, pp. 33422–33429.PubMedCrossRefGoogle Scholar
  2. Carpenter, G. and Cohen, S., Epidermal Growth Factor,Ann. Rev. Biochem., 1979, vol. 48, pp. 193–216.PubMedCrossRefGoogle Scholar
  3. Ganser, G.L., Stricklin, G.P., and Matrisian, L.M., EGF and TGF-u Influencein vitro Lung Development by the Induction of Matrix-Grading Metalloproteinases,Int. J. Devel. Biol., 1991, vol. 35, pp. 453–461.Google Scholar
  4. Haraguchi, S., Good, R.A., Engelman, R.W.,et al., Prolactin, Epidermal Growth Factor or Transforming Growth Factor α Activate a Mammary Tumor Virus-Long terminal Repeat,Molec. Cell Endocrinol., 1997, vol. 129, no. 2, pp. 145–155.PubMedCrossRefGoogle Scholar
  5. Jaskoll, T.F., Johonson, R., Don, G.,et al., Localization of Pulmonary Surfactant Protein During Mouse Lung Development,Devel. Biol., 1984, vol. 106, pp. 254–261.CrossRefGoogle Scholar
  6. Jaskoll, T.F., Johonson, R., Don, G.,et al., Embryonic Lung Morphogenesis in Serumless Chemically-Defined Mediumin vitro, Progress in Developmental Biology, Slavkin, H.C., Ed., New York: Alan R. Liss, 1986.Google Scholar
  7. Pache, J.C., Janssen, Y.M., Walsh, E.S.,et al., Increased Epidermal Growth Factor Receptor Protein in a Human Mesothelial Cell Line in response to Long Asbestos Fibers,Amer. J. Pathology, 1998, vol. 152, no. 2, pp. 333–340.Google Scholar
  8. Popova, N.V. and Rossi, L., The Expression of Transforming and Epidermal Growth Factors in Organ Cultures of Embryonic Lungs,Ontogenez, 1996, vol. 27, no. 6, pp. 440–444.PubMedGoogle Scholar
  9. Reddy, N., Everhart, A., Eling, T.,et al., Characterization of a 15-Lipoxygenase in Human Breast Carcinoma BT-20 Cell: Stimulation 13-HODE Formation by TGF Alpha/EGF,Biochem. Biophys. Res. Commun., 1997, vol. 231, no. 1, pp. 111–116.PubMedCrossRefGoogle Scholar
  10. Schuger, L., Skubitz, A.P.N., O’Shea, K.S.,et al., Identification of laminin Domains Involved in Branching Morphogenesis: Effects of Antilaminin Monoclonal Antibodies on Mouse Embryonic Lung Development,Dev. Biol., 1991, vol. 14, pp. 531–541.CrossRefGoogle Scholar
  11. Slavkin, H.C., Johnson, R., Oliver, P.,et al., Lamellar Body Formation Precedes Pulmonary Surfactant Apoprotein Expression During Embryonic Mouse Lung Developmentin vivo andin vitro, Differentiation, 1989, vol. 41, pp. 223–236.PubMedCrossRefGoogle Scholar
  12. Snead, M.L., Luo, W., Olizer, P.,et al., Localization of Epidermal Growth Factor Precursor in Tooth and Lung During Embryonic Mouse Development,Dev. Biol., 1989, vol. 134, pp. 420–429.PubMedCrossRefGoogle Scholar
  13. Ten Have-Opbroek, A.A.W., The Development of the Lung in Mammals: An Analysis of Concepts and Findings,Am. J. Anat., 1981, vol. 162, pp. 201–219.PubMedCrossRefGoogle Scholar

Copyright information

© MAIK “Nauka/Interperiodica” 2000

Authors and Affiliations

  • N. V. Popova
    • 1
  • L. Rossi
    • 2
  1. 1.Institute of Carcinogenesis, Blokhin Oncological Science CenterRussian Academy of Medical SciencesMoscowRussia
  2. 2.National Cancer InstituteGenoaItaly

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