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Russian Journal of Bioorganic Chemistry

, Volume 26, Issue 6, pp 381–389 | Cite as

Construction of a gene of the human tumor-associated antigen VNTR(MUC1) bound to streptavidin, its expression inEscherichia coli, and the study of properties of the hybrid protein

  • L. B. Gul’ko
  • O. V. Pavlova
  • N. A. D’yakov
  • N. A. Okorokova
  • K. I. Ratmanova
  • N. N. Logunova
  • R. A. Bobreneva
  • V. A. Makarov
  • V. L. Yurin
  • V. P. Veiko
  • V. G. Debabov
Article

Abstract

A gene of human tumor-associated antigen VNTR(MUC1) bound to streptavidin, an expression plasmid, and a highly effective hybrid protein-producing strain were constructed. It was shown that the streptavidin leader peptide ensures effective secretion of the hybrid protein into the periplasmic space ofEscherichia coli cells. The hybrid protein was isolated in a homogeneous state and its immunogenic properties were studied.

Key words

Escherichia coli hybrid protein MUC1 streptavidin tumor-associated antigen VNTR 

Abbreviations

KLH

snail hemocyanine

SAV

streptavidin

SAV(TR)22

the hybrid protein

TR

a tandem repeat monomer

TyR

bovine tyroglobulin

VNTR

the MUC1 protein locus with a variable number of tandem repeats; prefix “d” in deoxyribooligonucleotides is omitted

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Copyright information

© MAIK “Nauka/Interperiodica” 2000

Authors and Affiliations

  • L. B. Gul’ko
    • 1
  • O. V. Pavlova
    • 1
  • N. A. D’yakov
    • 1
  • N. A. Okorokova
    • 1
  • K. I. Ratmanova
    • 1
  • N. N. Logunova
    • 1
  • R. A. Bobreneva
    • 1
  • V. A. Makarov
    • 1
  • V. L. Yurin
    • 1
  • V. P. Veiko
    • 1
  • V. G. Debabov
    • 1
  1. 1.State Research Center of Genetics and Selection of Industrial Microorganisms of the Russian FederationMoscowRussia

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