Russian Journal of Bioorganic Chemistry

, Volume 26, Issue 7, pp 466–473 | Cite as

Specific features of enteropeptidase hydrolysis of chimeric proteins at the specific linker (Asp)4Lys depending on the refolding conditions

  • E. D. Shibanova
  • A. G. Mikhailova
  • S. L. Aleksandrov
  • L. D. Rumsh


Refolding from inclusion bodies of chimeric proteins containing the enteropeptidase-specific linker (Asp)4Lys was carried out. It was shown that, depending on the refolding conditions, chimeric proteins function as substrates or inhibitors of the enteropeptidase. The efficiency of the enteropeptidase hydrolysis of chimeric proteins containing the (Asp)4Lys linker may depend not only on the amino acid sequence of the protein binding site for the enzyme but also on the site’s conformation.

Key words

chimeric proteins folding limited proteolysis enteropeptidase 



protein of the VP1 region of the hepatitis A virus


protease of the human immunodeficiency virus


protease covalently joined with reverse transcriptase of the human immunodeficiency virus




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Copyright information

© MAIK “Nauka/Interperiodica” 2000

Authors and Affiliations

  • E. D. Shibanova
    • 1
  • A. G. Mikhailova
    • 1
  • S. L. Aleksandrov
    • 1
  • L. D. Rumsh
    • 1
  1. 1.Shemyakin-Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesGSP-7 MoscowRussia

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