Abstract
Induction of the expression of the 8-endotoxin gene fromBacillus thuringiensis var.tenebrionis in the recombinant strainPseudomonas putida IPM-36 negatively affected the viability and the growth rate of the culture. In order to optimize the insecticide production by the recombinant strain, mutant clones exhibiting anticipating growth on an inducer-containing medium were selected and studied. These clones differed in such aspects as the localization of mutations (either in plasmid pBTN11, carrying thecry3A gene, or in the chromosome), growth rate, or the level of δ-endotoxin synthesis after induction. Several obtained mutants proved much superior toP. putida IPM-36 in their structural and segregation stability, although they were as efficient as the original strain with respect to the production of the insecticide protein Cry3A.
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Koretskaya, N.G., Svetoch, O.E., Loseva, O.I. et al. Characterization of the mutant recombinant strainPseudomonas putida IPM-36 exhibiting anticipating growth on a medium containing an inducer of thecry3A gene expression. Microbiology 69, 559–564 (2000). https://doi.org/10.1007/BF02756808
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DOI: https://doi.org/10.1007/BF02756808