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Microbiology

, Volume 69, Issue 3, pp 271–276 | Cite as

Carbon metabolism inSulfobacillus thermosulfidooxidans subsp.asporogenes, strain 41

  • I. A. Tsaplina
  • E. N. Krasil’nikova
  • L. M. Zakharchuk
  • M. A. Egorova
  • T. I. Bogdanova
  • G. I. Karavaiko
Experimental Articles

Abstract

The activities of carbon metabolism enzymes were determined in cellular extracts of the moderately thermophilic, chemolithotrophic, acidophilic bacteriumSulfobacillus thermosulfidooxidans subsp.asporogenes, strain 41, grown either at an atmospheric content of CO2 in the gas phase (autotrophically, heterotrophically, or mixotrophically) or autotrophically at a CO2 content increased to 5–10%. Regardless of the growth conditions, all TCA cycle enzymes (except for 2-oxoglutarate dehydrogenase), one glyoxylate bypass enzyme (malate synthase), and some carboxylases (ribulose bisphosphate carboxylase, pyruvate carboxylase, and phosphoenolpyruvate carboxylase) were detected in the cell-free extracts of strain 411. During autotrophic cultivation of strains 41 and 1269, the increase in the CO2 content of the supplied air to 5–10% resulted in the activation of growth and iron oxidation, a 20–30% increase in the cellular content of protein, enhanced activity of the key TCA enzymes (citrate synthase and aconitase), and, in strain 41, a decrease in the activity of carboxylases.

Key words

thermoacidophiles growth TCA cycle enzymes carboxylases 

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Copyright information

© MAIK “Nauka/Interperiodica” 2000

Authors and Affiliations

  • I. A. Tsaplina
    • 1
  • E. N. Krasil’nikova
    • 2
  • L. M. Zakharchuk
    • 2
  • M. A. Egorova
    • 1
  • T. I. Bogdanova
    • 1
  • G. I. Karavaiko
    • 1
  1. 1.Russian Academy of SciencesInstitute of MicrobiologyMoscowRussia
  2. 2.Department of Microbiology,Biological FacultyMoscow State UniversityMoscowRussia

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