Comparative study of effects of cadmium cations in free and chelated forms on activity of glutathione S-transferase, growth, and endocytosis in culture of the infusoriumTetrahymena pyriformis
Effects of cadmium cations in free (Cd2+) and chelated with EDTA (Cd2+-EDTA) forms were studied on growth, endocytosis, and activity of glutathione S-transferase (GT) in the free-living infusoriaTetrahymena pyriformis. It is shown that the cytotoxicity of Cd2+ in the free form at a concentration of 10 μM is much higher than of the Cd2+-EDTA complex at the equimolar concentration. Even at a low concentration (2 μM), Cd2+ produces an inhibition of the growth rate and endocytosis in theT. pyriformis culture, while the Cd2+-EDTA complex suppresses these functions insignificantly. Cd2+ in the free form at concentrations of 10 and 100 μM reduced activity of glutathione S-transferase by 39 and 61%. The chelated Cd2+-EDTA complex at these concentrations inhibited the GT activity by 5 and 55%, respectively.
KeywordsCadmium Evolutionary Biochemistry Stationary Growth Phase Mercapturic Acid EDTA Complex
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