Comparative study of effects of cadmium cations in free and chelated forms on activity of glutathione S-transferase, growth, and endocytosis in culture of the infusoriumTetrahymena pyriformis
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Effects of cadmium cations in free (Cd2+) and chelated with EDTA (Cd2+-EDTA) forms were studied on growth, endocytosis, and activity of glutathione S-transferase (GT) in the free-living infusoriaTetrahymena pyriformis. It is shown that the cytotoxicity of Cd2+ in the free form at a concentration of 10 μM is much higher than of the Cd2+-EDTA complex at the equimolar concentration. Even at a low concentration (2 μM), Cd2+ produces an inhibition of the growth rate and endocytosis in theT. pyriformis culture, while the Cd2+-EDTA complex suppresses these functions insignificantly. Cd2+ in the free form at concentrations of 10 and 100 μM reduced activity of glutathione S-transferase by 39 and 61%. The chelated Cd2+-EDTA complex at these concentrations inhibited the GT activity by 5 and 55%, respectively.
KeywordsCadmium Evolutionary Biochemistry Stationary Growth Phase Mercapturic Acid EDTA Complex
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- 1.Nilsson, J.K., Tetrahymena in Cytotoxicology with Special Reference to Effects of Heavy Metals and Selected Drugs,Eur. J. Protistol., 1989, vol. 25, pp. 2–25.Google Scholar
- 5.Korotkov, S.M., Glasunov, V.V., Rozengart, E.V.,et al., Action of Organic Cadmium Complexes of Different Degree of Hydrophobicity on Rat Liver Mitochondria,Tsitologiya, 1996, vol. 38, pp. 1075–1083.Google Scholar
- 9.Pyne, C.K., The Effects of Cadmium on the Growth Pattern and Ultrastructure of the CiliateTetrahymena pyriformis, and Antagonistic Effect of Calcium,Biol. Cell, 1983, vol. 48, pp. 121–132.Google Scholar
- 10.Vasconcelos, S.D.M.T., Azenha, M.A.O., and Cabrai, J.P.S., Comparison of Availability of Copper (II) Complexes with Organic Ligands to Bacterial Cell and to Chitin,Microbiology, 1997, vol. 16, pp. 2029–2039.Google Scholar
- 13.Piccini, E., Irato, P., and Guidolin, L., Cadmium Thionein inTetrahymena thermophila andTetrahymena pyriformis, Eur. J. Protistol., 1990, vol. 26, pp. 176–181.Google Scholar