Abstract
Purpose
Our purpose was to compare the success rate of transferring frozen-thawed embryos generated from either intracytoplasmic sperm injection (ICSI) or conventional in vitro fertilization (IVF).
Methods
A retrospective review of all frozen—thawed embryo transfer (ET) cycles between January 1995 and April 1997 was performed. There were 83 and 204 transfer cycles of frozen — thawed multicellular embryos generated from conventional IVF (group A) and ICSI (group B), respectively. The survival rate of frozen — thawed embryos and the outcome following ET in both groups were assessed.
Results
The groups did not differ in age (31.7±4.6 and 30.6±6.0; mean±SD) or number of embryos transferred (3.5±1.1 and 3.8±1.3 for groups A and B, respectively). An acceptable pregnancy rate per ET was achieved in both groups, but the rate was significantly higher (P = 0.04) for group A than group B, 32.5 and 20%, respectively. Group A included frozen embryos of a higher quality than those of group B, but the proportion of embryos surviving after thawing was significantly higher for group B than group A (92.5 and 85.6%, respectively; P = 0.0004). The abortion rate did not differ between the two groups: 22 and 26.8% for groups A and B, respectively.
Conclusions
Although an overall high pregnancy rate was achieved following frozen-thawed ET, it was lower for cycles in which embryos had been generated from ICSI. This difference may be attributed to a lower prefreezing embryo quality in the ICSI group. Embryos originating from ICSI were not vulnerable to cryopreservation and, when implanted, resulted in a comparable abortion rate to thawed embryos of conventional IVF.
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References
Roest J, van Heusden AM, Verhoeff A, Mous HVH, Zeilmaker GH: A triplet pregnancy after in vitro fertilization is a procedure-related complication that should be prevented by replacement of two embryos only. Fertil Steril 1997;67:290–295
Trounson A: Cryopreservation. Br Med Bull 1990;46:695–708
Trounson A, Mohr L: Human pregnancy following cryopreservation. thawing and transfer of an eight-cell embryo. Nature 1983:305:707–709
Navot D, Bergh PA, Laufer N: Ovarian hyperstimulation syndrome in novel reproductive technologies: prevention and treatment. Fertil Steril 1992:58:249–261
Van Steirteghem AC, Liu J, Joris H, Nagy Z, Janssenswillen C, Tournaye H,et al.: Higher success rate by intracytoplasmic sperm injection than by subzonal insemination. Report of a second series of 300 consecutive treatment cycles. Hum Reprod 1993:8:1055–1060
Palermo GD, Cohen J, Alikani M, Adler A, Rosenwaks Z: Intracytoplasmic sperm injection: A novel treatment for all forms of male factor infertility. Fertil Steril 1995:63:1231–1240
Van Steirteghem AC, Van der Elst J, Van den Abbeel E, Joris H, Camus M, Devroey P: Cryopreservation of supranumerary multicellular human embryos obtained after intracytoplasmic sperm injection. Fertil Steril 1994:62:775–780
Al-Hasani S, Ludwig M, Gagstreiger F, Klipker W, Sturm R, Yilmaz A,et al: Comparison of cryopreservation of supranumerary pronuclear human oocytes obtained after intracytoplasmic sperm injection (ICSI) and after conventional in-vitro fertilization. Hum Reprod 1996:11:604–607
Hoover L, Baker A, Check JH, Lurie D, Summers D: Clinical outcome of cryopreserved human pronuclear stage embryos resulting from intracytoplasmic sperm injection. Fertil Steril 1997:67:621–624
Lin YP, Cassidenti DL, Chacon RR, Soubra SS, Rosen GF, Yee B: Successful implantation of frozen sibling embryos is influenced by the outcome of the cycle from which they were derived. Fertil Steril 1995:63:262–267
Catt JW, Ryan JP, Pike IL, O’Neill C, Saunders DM: Clinical intracytoplasmic sperm injection (ICSI) results from Royal North Shore. Reprod Fertil Dev 1995:7:255–262
Bourne H, Richings N, Harari O, Watkins W, Speirs AL, Johnston WI,et al: The use of intracytoplasmic sperm injection for the treatment of severe and extreme male infertility. Reprod Fertil Dev 1995:7:237–245
Payne D, Matthews CD: Intracytoplasmic sperm injection — Clinical results from the reproductive medicine unit, Adelaide. Reprod Fertil Dev 1995:7:219–227
Simon A, Benshushan A, Shushan A, Zajicek G, Dorembus D, Lewin A, Laufer N: A comparison between a standard and reduced dose of D-Trp-6-luteinizing hormone-releasing hormone administered after pituitary suppression for in-vitro fertilization. Hum Reprod 1994:9:1813–1817
Testart J, Lassalle B, Belaisch-Allart J, Hazout A, Forman R, Rainborn JD,et al.: High pregnancy rate after early human embryo freezing. Fertil Steril 1986:46:268–272
Society for Assisted Reproductive Technology, American Society for Reproductive Medicine. Assisted reproductive technology in the United States and Canada: 1994 results generated from the American Society for Reproductive Medicine/Society for Assisted Reproductive Technology Registry. Fertil Steril 1996:66:697–705
Camus M, Van den Abbeel E, Van Waesberghe L, Wisanto A, Devroey P, Van Steirteghem AC: Human embryo viability after freezing with dimethylsulphoxide as a cryoprotectant. Fertil Steril 1989:51:460–465
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Simon, A., Holzer, H., Hurwitz, A. et al. Comparison of cryopreservation outcome following intracytoplasmic sperm injection and conventional in vitro fertilization. J Assist Reprod Genet 15, 431–437 (1998). https://doi.org/10.1007/BF02744937
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DOI: https://doi.org/10.1007/BF02744937