Cell adhesion and growth on synthetic hydrogel beads
Hydrolysed poly(methyl methacrylate) microspheres with carboxyl residues distributed throughout the matrix were tested for their ability to support cell adhesion and growth. Cell growth as determined by protein content, phase contrast and scanning electron microscopy showed that these microspheres are growth-supportive. Further, preliminary experiments pointed to their usefulness in microcarrier culture.
KeywordsCell adhesion cell growth synthetic hydrogel beads hydrolysed poly(methyl methacrylate) microspheres microcarrier culture
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- Hirtenstein M, Clark J, Lindgren G and Vretblad D 1980Dev. Biol. Standard 46 109Google Scholar
- Jayakrishnan A, Chitambara Thanoo B, Rathinam K, Mandalam K R, Rao V R K, Lal A V and Mohanthy M 1989Bull. Mater. Sci. 12 17Google Scholar
- Johansson A and Nielsen V 1980Dev. Biol. Stand. 46 125Google Scholar
- Lowry O H, Rosebrough N J, Farr A L and Randall R J 1951J. Biol. Chem. 193 265Google Scholar
- Meignier B, Mongeot H and Farre H 1980Dev. Biol. Stand. 46 249Google Scholar
- Montagnon B J, Fanget B and Nicolas A J 1981Dev. Biol. Stand. 47 55Google Scholar
- Page M and Dufour C 1979J. Cell. Biol. 83 A302Google Scholar
- Rosen J J and Schway M B 1979ACS Organic Coating and Plastics Chemistry 40 636Google Scholar
- Van Wezel A L, van der Velden, de Groot C A M and van Herwaarden J A N 1980Dev. Biol. Stand. 46 151Google Scholar