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Applied Biochemistry and Microbiology

, Volume 36, Issue 3, pp 282–288 | Cite as

Preparation of conjugated antigens based on zearalenone carboxymethyloxime and their use in enzyme immunoassay

  • A. A. Burkin
  • G. P. Kononenko
  • N. A. Soboleva
  • E. V. Zotova
Article

Abstract

Zearalenone-6′-carboxymethyloxime was synthesized, and its conjugates to albumins and gelatin were prepared. Polyclonal rabbit antibodies against the conjugate with bovine serum albumin were shown to be highly specific to zearalenone and to have a lower cross-reactivity toward its structural analogues (a-zearalenol, 28%; ß-zearalenol, 6%; zearalanone, 12%; and α-zearalanol, 5%). The sensitivity of enzyme immunoassay with immobilized gelatin-based conjugates for determination of zearalenone in solution was 1 ng/ml, and this allowed us to determine this substance in feed at a threshold concentration of 200 μg/kg.

Keywords

Apply Biochemistry Zearalenone Bovine Serum Albu Rabbit Serum Albumin Epitope Density 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© MAIK “Nauka/Interperiodica” 2000

Authors and Affiliations

  • A. A. Burkin
    • 1
  • G. P. Kononenko
    • 1
  • N. A. Soboleva
    • 1
  • E. V. Zotova
    • 1
  1. 1.All-Russia Institute of Veterinary Sanitation, Hygiene, and EcologyRussian Academy of Agricultural SciencesMoscowRussia

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