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Ascorbat- und Glutathiongehalt in verschiedenartig schadstoffbeeinflußten Nadeln vonPinus sylvestris L.

Ascorbate and glutathione contents in diversely pollutant affected needles ofPinus sylvestris L.

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Zusammenfassung

In Extrakten von Nadelmischproben (Pinus sylvestris L.) wurde der Gesamtgehalt von Ascorbat und Glutathion mittels HPLC-Methoden bestimmt. Dabei konnte die chromatographische Abtrennung von Glutathion durch Optimierung des Gradientenprofils verbessert werden.

Die Untersuchungen erfolgten an Nadelproben aus Kiefernjung- und-altbeständen mit differenzierter SO2-Immission. Mit zunehmender Immissionsbelastung zeigten diesjährige Nadeln von Jungbeständen einen Anstieg im Gesamtascorbatgehalt. Der gleiche Effekt wurde für den Gesamtglutathiongehalt sowohl in Jung- als auch in Altbeständen beobachtet. Diese Befunde wurden mit Literaturangaben anderer Untersuchungssysteme (Versuche in Expositions- und Open-Top-Kammern) diskutiert.

Summary

Concentrations of antioxidants ascorbate and glutathione in Scots pine needles (Pinus sylvestris L.) taken from trees in the field were determined using the HPLC technique. A modification of the gradient profile made it possible to achieve a better separation between glutathione and the derivatization reagent monobromobimane in the chromatogram.

The needles were taken from pine trees 25 years and 60 years old, growing in areas with varying pollution. This year's needles of young pine trees were characterized by an increase in the total ascorbate content related to rising amounts of atmospheric pollutants. A similar trend was observed for glutathione in both young and old trees. These results are discussed in comparison with literature data on related open-top chamber experiments.

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  1. Sektion Chemische Ökotoxikologie, UFZ-Umweltforschungszentrum Leipzig-Halle GmbH, Hallesche Straße 44, D-06246, Bad Lauchstädt

    Sigrid Härtling & H. Schulz

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  1. Sigrid Härtling
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  2. H. Schulz
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Härtling, S., Schulz, H. Ascorbat- und Glutathiongehalt in verschiedenartig schadstoffbeeinflußten Nadeln vonPinus sylvestris L.. Forstw Cbl 114, 40–49 (1995). https://doi.org/10.1007/BF02742210

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