Molecular Biotechnology

, Volume 6, Issue 2, pp 201–211 | Cite as

Primedin situ labeling (PRINS)

A fast method forin situ labeling of nucleic acids
  • Johnny Hindkjær
  • Jørn Koch
  • Carsten Brandt
  • Steen Kølvraa
  • Lars Bolund


PRimedIn Situ labeling (PRINS) is a fast and sensitive alternative to fluorescencein situ hybridization (FISH) for identification of chromosome aberrations. In this article, we present the detailed protocols for detection of repeat sequences using oligonucleotides or fragments of cloned probes as primers for PRINS. We describe a multicolor PRINS procedure for simultaneous visualization of more probes in different colors on a metaphase preparation, and a PRINS-painting procedure, which combines PRINS and chromosome painting. Finally, a protocol for detection of single-copy genes is presented.

Index Entries

PRINS oligonucleotide primers in situ hybridization chromosome aberrations 


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Copyright information

© Humana Press Inc 1996

Authors and Affiliations

  • Johnny Hindkjær
    • 1
  • Jørn Koch
    • 1
  • Carsten Brandt
    • 1
  • Steen Kølvraa
    • 1
  • Lars Bolund
    • 1
  1. 1.Department of Gynecology and ObstetricsÅrhus University HospitalÅrhus NDenmark

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