Endocrine Pathology

, Volume 9, Issue 3, pp 195–199 | Cite as

Catalyzed reporter deposition method for amplifying endocrine products



The catalyze reporter deposition (CARD) method is a new technique to enhance signal detection in immunostaining, immunoelectron microscopy, andin situ hybridization. This technique, which uses a biotinylated tyramine amplification reagent, has been applied to endocrine cells and tumors, and has shown a marked increase in sensitivity in detecting small amounts of protein antigens in endocrine cells and tumors. Although some technical problems remain before widespread application of this method can be used, the potential for more in-depth understanding of normal and neoplastic endocrine tissue by CARD analysis is largely unexplored at this time.

Key Words

Immunohistochemistry endocrine tissue signal amplification biotinylated tyramine 


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  1. 1.
    Sternberger LA, Hardy PH Jr, Cuculis JJ, Meyer HG. The unlabeled antibody enzyme method of immunocytochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-anti peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18: 315–333, 1970.PubMedGoogle Scholar
  2. 2.
    Hsu SM, Raine L, Fanger H. A comparative study of the peroxidase-antiperoxidase method and an avidin-biotin complex method for studying polypeptide hormones with radioimmunoassay antibodies. Am J Clin Pathol 75:734–738, 1981.PubMedGoogle Scholar
  3. 3.
    Merz H, Malisius R, Mannweiler S, Zhou R, Hartmann W, Orscheschek K, Moubayerd P, Feller AC. ImmunoMax: a maximized immunohistochemical method for the retrieval and enhancement of hidden antigens. Lab Invest 73:149–156, 1995.PubMedGoogle Scholar
  4. 4.
    Shi SR, Key ME, Kalra KL. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem 39:741–748, 1991.PubMedGoogle Scholar
  5. 5.
    Bobrow, MN, Harris, TD, Shaughnessy KJ, Litt GJ. Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays. J Immunol Method 125:279–285, 1989.CrossRefGoogle Scholar
  6. 6.
    Adams JC. Biotin amplification of biotin and horseradish peroxidase signals in histochemical stains. J Histochem Cytochem 40:1457–1463, 1992.PubMedGoogle Scholar
  7. 7.
    Sanno N, Teramoto A, Sugiyama M, Itoh Y, Osamura RY. Application of catalyzed signal amplification in immunodetection of gonadotropin subunits in clinically nonfunctioning pituitary adenomas. Am J Clin Pathol 106: 16–21, 1996.PubMedGoogle Scholar
  8. 8.
    Mayer G, Bendayan M. Biotinyl-tyramide: a novel approach for electron microscopic immunocytochemistry. J Histochem Cytochem 45:1449–1454, 1997.PubMedGoogle Scholar
  9. 9.
    Kerstens HM, Poddighe PJ, Hanselaar AJM. A novel in situ hybridization signal amplification method based on the deposition of biotinylated tyramine. J Histochem Cytochem 43:347–352, 1995.PubMedGoogle Scholar
  10. 10.
    von Wasielewski R, Mengel M, Gignac S, Wilkens L, Werner M, Georgii A. Tyramine amplification technique in routine immunohistochemistry 45:1455–1459, 1997.Google Scholar
  11. 11.
    Hunyady B, Krempels K, Harta G, Mezey E. Immunohistochemical signal amplification by catalyzed reporter deposition and its application in double immunostaining. J Histochem Cytochem 44:1353–1362, 1996.PubMedGoogle Scholar
  12. 12.
    von Gijlswijk RP, Zijlmans HJ, Wiegant J, Bobrow MN, Erickson TJ, Adler KE, Tanke HJ, Raap AK. Fluorochrome-labeled tyramides: use in immunohistochemistry and fluorescence in situ hybridization. J Histochem Cytochem 45:375–382, 1997.PubMedGoogle Scholar
  13. 13.
    Koji T, Kanemitsu Y, Hoshino A, Nakane PK. A novel amplification method of nonradioactive in situ hybridization signal for specific RNA with biotinylated tyramine. Acta Histochem Cytochem 30:401–406, 1997.Google Scholar

Copyright information

© Humana Press Inc 1998

Authors and Affiliations

  1. 1.Department of NeurosurgeryNippon Medical SchoolTokyoJapan
  2. 2.Department of PathologyTokai University School of MedicineIsehara-city KanagawaJapan

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