, Volume 3, Issue 7, pp 519–524 | Cite as

The LIM-homeodomain protein Isl-1 segregates with somatostatin but not with gastrin expression during differentiation of somatostatin/gastrin precursor cells

  • Lars-Inge Larsson
  • Jens-Erik Tingstedt
  • Ole D. Madsen
  • Palle Serup
  • David M. Hougaard


The gastric epithelium is renewed from stem cells in the isthmus of the gastric glands. We describe that the two neuroendocrine peptides gastrin and somatostatin are coexpressed by isthmic stem cells. Bromodeoxyuridine labeling indicates that the coexpressing cells divide and differentiate into gastrin and somatostatin cells, which remain in paracrine contact during most of their migration down into the gland. The coexpressing cells display nuclear immunoreactivity for the transcription factors Isl-1 and CREB, which have been implicated in somatostatin gene expression. Differentiated gastrin cells lack Isl-1 reactivity and show variable staining for CREB while differentiated somatostatin cells display Isl-1 and CREB reactivity.


somatostatin gastrin coexpression Isl-1 homeobox CREB 


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Copyright information

© Humana Press Inc 1995

Authors and Affiliations

  • Lars-Inge Larsson
    • 1
  • Jens-Erik Tingstedt
    • 1
  • Ole D. Madsen
    • 2
  • Palle Serup
    • 2
  • David M. Hougaard
    • 1
  1. 1.Department of Molecular Cell Biology, Biotechnology DivisionStatens SeruminstitutCopenhagen SDenmark
  2. 2.Hagedorn Research InstituteGentofteDenmark

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