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DNA Polymerase-associated and autonomous vertebrate 3′→5′ exonuleases

  • Comparative and Ontogenic Biochemistry
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Abstract

Using methods of gel filtration and ultracentrifugation, cell-free extracts from 12 objects representing the main vertebrate representatives (bony fish, amphibian, reptiles, birds, mammals, including human) were studied. The enzyme activity of autonomous 3′→5′-exonucleases (AE) has been established to be 25–90% of the total 3′→5′ exonuclease activity of the extracts. A part of the AE is revealed in a zone of the DNA polymerases of the α-family and can be separated by changing Chromatographic conditions or by repeated fractionation. The high activity of AE allows suggesting their substantial participation in the replicative correction of the DNA-poly-merase errors as well as in the postreplicative correction of the heteroduplexes in the vertebrate DNA.

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Authors and Affiliations

  1. Laboratory of DNA Synthesis, Department of Molecular and Radiation Biophysics, St. Petersburg Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Russia

    N. L. Ronzhina, T. P. Kravetskaya & V. M. Krutyakov

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  1. N. L. Ronzhina
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  2. T. P. Kravetskaya
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  3. V. M. Krutyakov
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Ronzhina, N.L., Kravetskaya, T.P. & Krutyakov, V.M. DNA Polymerase-associated and autonomous vertebrate 3′→5′ exonuleases. J Evol Biochem Phys 36, 262–266 (2000). https://doi.org/10.1007/BF02737041

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  • DOI: https://doi.org/10.1007/BF02737041

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