DNA Polymerase-associated and autonomous vertebrate 3′→5′ exonuleases
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Using methods of gel filtration and ultracentrifugation, cell-free extracts from 12 objects representing the main vertebrate representatives (bony fish, amphibian, reptiles, birds, mammals, including human) were studied. The enzyme activity of autonomous 3′→5′-exonucleases (AE) has been established to be 25–90% of the total 3′→5′ exonuclease activity of the extracts. A part of the AE is revealed in a zone of the DNA polymerases of the α-family and can be separated by changing Chromatographic conditions or by repeated fractionation. The high activity of AE allows suggesting their substantial participation in the replicative correction of the DNA-poly-merase errors as well as in the postreplicative correction of the heteroduplexes in the vertebrate DNA.
KeywordsEvolutionary Biochemistry Nuclease Activity Exonuclease Activity Repeated Chromatography Substantial Participation
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- 1.Krutyakov, V.M., Rational Regulation of DNA-Polymerase Mutagenesis and Autonomous 3′→5′-Exonucleases,Mol. Biol., 1998, vol. 32, pp. 229–232.Google Scholar
- 2.Krutyakov, V.M., Accuracy of DNA Synthesis: The Corrector Role of Mammalian Autonomous 3′→5′-Exonucleases,Usp. Sovrem. Biol., 1997, vol. 117, pp. 660–667.Google Scholar
- 11.Legina, O.K., Belyakova, N.V., Kleiner, N.E.,et al., Homogenous 3′→5′ Exonucleases and Their Multienzyme Complexes from the Rat Liver,Mol. Biol., 1990, vol. 24, pp. 156–162.Google Scholar