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Journal of Molecular Neuroscience

, Volume 4, Issue 1, pp 21–28 | Cite as

Chronic treatment of newborn rats with naltrexone alters astrocyte production of nerve growth factor

  • Kunihiko Mitsuo
  • Joan P. Schwartz
Article

Abstract

Newborn rats were treated with the opiate antagonist naltrexone daily for 1–2 wk in order to examine the effects of endogenous opioid peptides on astrocytes during CNS development. Nerve growth factor (NGF) and cyclic AMP were measured in astrocytes cultured from cerebellum, striatum, and hippocampus of 1 d, 1 wk, and 2 wk postnatal rats. Cerebellar and striatal, but not hippocampal, astrocytes prepared from naltrexone-treated animals produced higher levels of NGF than those from controls. The turnover rate of cyclic AMP, measured following treatment of the cells with forskolin in the presence of the phosphodiesterase inhibitor IBMX, was increased in naltrexone-derived cerebellar and striatal astrocytes. Opiate receptors could not be detected on the cultured astrocytes, either by direct binding of3H-etorphine or by modulation of cyclic AMP content. These results suggest that endogenous opioid peptides may function indirectly to alter trophic factor synthesis in astrocytes.

Index Entries

Opioid peptides cyclic AMP opiate receptors CNS development 

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Copyright information

© Humana Press, Inc 1993

Authors and Affiliations

  • Kunihiko Mitsuo
    • 1
  • Joan P. Schwartz
    • 1
  1. 1.Clinical Neuroscience Branch, National Institute of Neurological Disorders and StrokeNational Institutes of HealthBethesda

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