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Applied Biochemistry and Microbiology

, Volume 36, Issue 5, pp 496–501 | Cite as

Comparative immunochemical characterization of polyclonal and monoclonal antibodies to aflatoxin B1

  • M. A. Burkin
  • I. V. Yakovleva
  • V. V. Sviridov
  • A. A. Burkin
  • G. P. Kononenko
  • N. A. Soboleva
Article

Abstract

Four hybrid clones (MM-(AB1)-1, MM-(AB1)-2, MM-(AB1)-3, and MM-(AB1)-4) were obtained by hybridoma technology involving the immunization of BALB/c mice with a BSA conjugate of aflatoxin B1 carboxymethyloxime derivative. Antibodies produced by these clones varied in their ability to recognize the aflatoxin B1 analogues. The sensitivity of enzyme immunoassay based on all monoclonal antibodies was higher compared to analysis based on polyclonal rabbit antibodies (0.1 and 0.4 ng/ml, respectively).

Keywords

Apply Biochemistry Aflatoxin Mold Fungus ELISA Condition Immune Reagent 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© MAIK “Nauka/Interperiodica” 2000

Authors and Affiliations

  • M. A. Burkin
    • 1
  • I. V. Yakovleva
    • 1
  • V. V. Sviridov
    • 1
  • A. A. Burkin
    • 2
  • G. P. Kononenko
    • 2
  • N. A. Soboleva
    • 2
  1. 1.Russian Academy of Medical SciencesMechnikov Institute of Vaccines and SeraMoscowRussia
  2. 2.Hygiene, and Ecology, Russian Academy of Agricultural SciencesAll-Russia Institute of Veterinary SanitationMoscowRussia

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