Skip to main content
Log in

Microbial growth on pall rings

A problem when upgrading biogas with the water-wash absorption technique

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Biogas is upgraded using an absorption with water-wash technique by 11 of a total of 14 upgrading plants in Sweden. However, problems with microbial growth on the pall rings in the absorption column, and in one case in the desorption column, have a negative impact on the upgrading of raw gas to vehicle gas. Five of the nine biogas plants studied here have experienced problems with microbial growth. The objectives of this study were to identify such microbial growth and to determine possible factors for its control, in order to provide recommendations for process management. A questionnaire was sent out and visits were made to the upgrading plants to collect information about the upgrading process. Phospholipid fatty acid (PLFA) analysis was performed to determine microbial biomass and community structure in samples from four upgrading plants. In samples from two of the plants, methane-oxidizing bacteria (type I methanotrophs) were indicated, while samples from one of the other plants showed biomarkers indicating actinomycetes. Factors affecting development of microbial growth were found to be water quality and the pH and temperature of the process water. Plants that used wastewater in the upgrading process experienced far more problems than those using clean water of drinking quality.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. SGC (Swedish Gas Centre). (2001), Report SGC 118, SGC, Scheelegatan 3, SE-212 28 Malmö, Sweden.

  2. Flotech. (2004), Technical Report, 2004. Flotech Sweden, Stockholm, Sweden.

  3. Persson, M. (2003), MSc thesis, Lund Technical University, Lund, Sweden. (Also available as Report SGC 142, Swedish Gas Centre, Scheelegatan 3, SE-212 28 Malmö, Sweden, 2003).

  4. Pinkart, H. C., Ringelberg, D. B., Piceno, Y. M., Macnaughton, S. J., and White, D. C. (1999), inManual of Environmental Microbiology, 2nd ed., Hurst, C. H., Crawford, R. L., Knudsen, G., McInerney, M., and Stetzenbach, L. D., eds., American Society for Microbiology Press, Washington, DC, pp. 101–113.

    Google Scholar 

  5. Hilger, H. A., Cranford, D. F., and Barlaz, M. A. (2000),Soil Biol. Biochem. 32, 457–467.

    Article  CAS  Google Scholar 

  6. Börjesson, G., Sundh, I., Tunlid, A., and Svensson, B. H. (1998),Soil Biol. Biochem. 30, 1423–1433.

    Article  Google Scholar 

  7. Börjesson, G., Sundh, I., and Svensson, B. H. (2004),FEMS Microbiol. Ecol. 48, 305–312.

    Article  CAS  Google Scholar 

  8. Steger, K., Jarvis, A., Smąrs, S., and Sundh, I. (2003),J. Microbiol. Methods 55, 371–382.

    Article  CAS  Google Scholar 

  9. Frostegąrd, A., Tunlid, A., and Bąąth, E. (1993),Soil Biol. Biochem. 25, 723–730.

    Article  Google Scholar 

  10. Bąąth, E. and Andersson, T. H. (2003),Soil Biol. Biochem. 35, 955–963.

    Article  CAS  Google Scholar 

  11. McNabb, A., Shuttleworth, R., Behme, R., and Colby, W. D. (1997),J. Clin. Microbiol. 35, 1361–1368.

    CAS  Google Scholar 

  12. Guckert, J. B., Antworth, C. P., Nichols, P. D., and White, D. C. (1985),FEMS Microbiol. Ecol. 31, 147–158.

    CAS  Google Scholar 

  13. Bowman, J. P. (2006), inThe Prokaryotes: An Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, K-H., and Stackenbrandt, E., eds., Springer-Verlag, New York, Part 1, Section 3, pp. 266–289.

    Chapter  Google Scholar 

  14. Gordienko, A. S., Kurdish, I. K., and Kisten, A. G. J. (1997),Water Chem. Technol. 19, 40–43.

    Google Scholar 

  15. Huq, M. N., Ralph, B. J., and Rickard, P. A. D. (1978),Aust. J. Biol. Sci. 31, 311–316.

    CAS  Google Scholar 

  16. Wilshusen, J. H., Hettiaratchi, J. P. A., De Visscher, A., and Saint-Fort, R. (2004),Environ. Pollut. 129, 305–314.

    Article  CAS  Google Scholar 

  17. Wilshusen, J. H., Hettiaratchi, J. P. A., and Stein, V. B. (2004),Waste Management 24, 643–653.

    Article  CAS  Google Scholar 

  18. Fassel, T. A., Vanover, J. E., Hauser, C. C., Buchholz, L. E., Edmiston, C. E., Sanger, J. R., and Remsen, C. C. (1992), Cells Mater.2, 37–48.

    Google Scholar 

  19. Lechevalier, M. P. and Lechevalier, H. A. (1974),Int. J. Syst. Bacteriol. 24, 278–288.

    Article  Google Scholar 

  20. Lechevalier, H. A. (1975), Actinomycetes of sewage treatment plants. Environmental Protection Technology Series Report, EPA-600/2-75-031, US Environmental Protection Agency. P.O. Box 42419, Cincinatti, OH, 1975.

  21. Lemmer, H. (1986),Water Res. 20, 531–535.

    Article  Google Scholar 

  22. Dizer, H. and Hagendorf, U. (1991),Water Res. 25, 791–796.

    Article  CAS  Google Scholar 

  23. Reponen, T., Gazenko, S. V., Grinshpun, S. A., Willeke, K., and Cole, E. C. (1998),Appl. Environ. Microbiol. 64, 3807–3812.

    CAS  Google Scholar 

  24. Oppong, D., King, V. M., Zhou, X., and Bowen, J. (2000),Ind. Microbiol. Biotechnol. 25, 74–80.

    Article  CAS  Google Scholar 

  25. Safade, T. L. (1988),Paper Technol. Ind. 29, 280–285.

    CAS  Google Scholar 

  26. Blackall, L. L., Seviour, E. M., Bradford, D., Stratton, H. M., Cunningham, M. A., Hugenholtz, P., and Seviour, R. J. (1996),Water Sci. Technol. 34, 137–144.

    Article  CAS  Google Scholar 

  27. Käppala Industries. (2001), http://www.kappala.se/admin/varor/docs/miljorapporter2001.pdf. Ac-cessed October 17, 2005.

  28. Andersson, F. A. (2003), Evaluation of detergents used for dissolving microbial growth on pall rings. PM 2003-11-05. Linköping: Tekniska Verken.

    Google Scholar 

  29. Adamsen, A. P. S. and King, G. M. (1993),Appl. Environ. Microbiol. 59, 485–490.

    CAS  Google Scholar 

  30. Saari, A., Rinnan, R., and Martikainen, P. (2004),Soil Biol. Biochem. 36, 1037–1046.

    Article  CAS  Google Scholar 

  31. King, G. M. (1990),FEMS Microbiol. Lett. 74, 309–323.

    CAS  Google Scholar 

  32. McDonald, I. R., Hall, G. H., Pickup, R. W., and Murrell, J. C. (1996),FEMS Microbiol. Ecol. 21, 197–211.

    Article  CAS  Google Scholar 

  33. CDM (2005), Foamaster ENA-389. Product Information. In Swedish. http://www.cdm.se. Accessed January 17, 2005.

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Gunnar Börjesson.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tynell, Å., Börjesson, G. & Persson, M. Microbial growth on pall rings. Appl Biochem Biotechnol 141, 299–319 (2007). https://doi.org/10.1007/BF02729069

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02729069

Index Entries

Navigation