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Two-dimensional gel analysis of proteins in cell lines from the central nervous system of larvalDrosophila

  • Juan F. Santarén
Growth, Differentiation and Senescence

Summary

High resolution two-dimensional gel electrophoresis was used to quantitatively analyze the patterns of protein synthesis in three different clones of a nerve cell line (ML-DmBG2) ofDrosophila melanogaster. When patterns of pulse-labeled proteins of the three different clones were compared, I observed quantitative variations affecting the rate of synthesis by twofold or more in 25–30% of the polypeptides and qualitative differences, always affecting less than 2% of the polypeptides. Patterns of protein synthesis were analyzed during the 24 d of culture, revealing both quantitative (increase or decrease; 40%) and qualitative (presence or absence; 3%) differences. More than 70 proteins synthesized in these cultures were secreted into the medium. Among them were two major groups of acidic proteins which disappeared with culture time. When cell lines and intact central nervous systems were compared, large differences in protein synthesis were observed. In fact, only 20% of the synthesized proteins were common to both isolated cells grownin vitro and the original nervous systemin vivo.

Key words

Drosophila central nervous system 2-D gels cell lines 

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Copyright information

© Society for In Vitro Biology 1996

Authors and Affiliations

  • Juan F. Santarén
    • 1
  1. 1.Centro de Biología Molecular “Severo Ochoa,” CSIC-UAMUniversidad Autónoma de MadridMadridSpain

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