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Establishment and characterization of a novel human myoepithelial cell line and matrix-producing xenograft from a parotid basal cell adenocarcinoma

  • Mark D. Sternlicht
  • Suzi Safarians
  • Thomas C. Calcaterra
  • Sanford H. Barsky
Cellular Models

Summary

Myoepithelial cells exert important paracrine effects on epithelial morphogenesis and mitogenesis through direct cell-cell interactions and through synthesis of a basement membrane extracellular matrix. To study these effects further, this study established the first immortalized human myoepithelial cell line, HMS-1, and transplantable xenograft, HMS-X, from the rare parotid basal cell adenocarcinoma. The cell line exhibited a fully differentiated myoepithelial phenotype and the xenograft exhibited the rare property of accumulating an abundant extracellular matrix composed of both basement membrane and nonbasement membrane components with the latter predominating. With HMS-1 as a feeder layer, dramatic and specific induction of epithelial morphogenesis (sheroid formation) occurred with selected normal epithelial and primary carcinoma target cells. HMS-1 and HMS-X provide distinct advantages over the conventional murine matrices in existence. They will be invaluable in future studies of human tumor-myoepithelial and matrix interactions important for tumor cell growth, invasion, and metastasis.

Key words

salivary gland tumors myoepithelial cells extracellular matrix morphogenesis 

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Copyright information

© Society for In Vitro Biology 1996

Authors and Affiliations

  • Mark D. Sternlicht
    • 1
  • Suzi Safarians
    • 1
  • Thomas C. Calcaterra
    • 2
  • Sanford H. Barsky
    • 1
  1. 1.Department of PathologyUCLA School of MedicineLos Angeles
  2. 2.Department of Surgery, Head and Neck DivisionUCLA School of MedicineLos Angeles

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