Mycoplasma orale infection affects K+ and Cl− currents in the HSG salivary gland cell line
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The relations between K+ channel and Cl− channel currents and mycoplasma infection status were studied longitudinally in HSG cells, a human submandibular gland cell line. The K+ channel currents were disrupted by the occurrence of mycoplasma infection: muscarinic activation of K+ channels and K+ channel expression as estimated by ionomycin- or hypotonically induced K+ current responses were all decreased. Similar decreases in ionomycin- and hypotonically induced responses were observed for Cl− channels, but only the latter decrease was statistically significant. Also, Cl− currents could be elicited more frequently than K+ currents (63% of cases versus 0%) in infected cells when tested by exposure to hypotonic media, indicating that mycoplasma infection affects K+ channels relatively more than Cl− channels. These changes occurred in the originally infected cells, were ameliorated when the infection was cleared with sparfloxacin, and recurred when the cells were reinfected. Such changes would be expected to result in hyposecretion of salivary fluid if they occurredin vivo.
Key wordspotassium channels chloride channels submandibular gland infection
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- 1.Ahmed, M. A. Curing mycoplasma in tissue culture with two newer quinolones: sparfloxacin and OPC17116 [MSc. thesis]. Seattle: University of Washington; 1993.Google Scholar
- 3.Bauer, F. A.; Wear, D. J.; Angritt, P., et al.Mycoplasma fermentans (incognitus strain) infection in the kidneys of patients with acquired immunodeficiency syndrome and associated nephropathy: a light microscopic, immunohistochemical and ultrastructural study. Human Pathol. 22:932–933; 1991.Google Scholar
- 8.Clyde, W. A. Mycoplasma species identification based upon growth inhibition by specific antisera. J. Immunol. 92:955–965; 1964.Google Scholar
- 15.Kenny, G. E. Manual of clinical microbiology. In: Balows, A.; Hausler, W. J., et al., ed. Washington, DC: American Society for Microbiology; 1991:478–482.Google Scholar
- 16.Kenny, G. E.; Cartwright, F. D. Susceptibility ofMycoplasma pneumoniae to several new quinolones, tetracycline, and erythromycin. Antimicrob. Agents Chemother. 35:587–589; 1991.Google Scholar
- 18.Lau, K. R.; Howorth, A. J.; Case, R. M. The effects of bumetanide, amiloride and Ba2+ on fluid and electrolyte secretion in rabbit salivary gland. J. Physiol. (Lond.) 425:407–427; 1990.Google Scholar
- 23.Patton, L. L.; Wellner, R. B. Established salivary cell lines. In: Dobrosielski-Vergona, K., ed. Biology of the salivary glands. Boca Raton: CRC Press; 1993:319–341.Google Scholar
- 24.Pollack, J. D.; Jones, M. A.; Williams, M. V. The metabolism of AIDS-associated mycoplasmas. Clin. Infect. Dis. 17:S267–271; 1993.Google Scholar
- 28.Schiodt, M.; Greenspan, D.; Levy, J. A., et al. Does HIV cause salivary gland disease? J. Acquired Immune Defic. Syndr. 3:819–822; 1989.Google Scholar
- 30.Siegel, S. Nonparametric statistics. New York: McGraw-Hill; 1956: 96 p.Google Scholar