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Enzyme activities of lung lavage in silicosis

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Abstract

The cytotoxic effect of quartz on lung cells has been well documented by in vitro and animal studies, but the pertinence of these findings to humans has not yet been documented. We measured lactate dehydrogenase (LDH) activities in the lung lavage of 24 long-term workers in the Québec granite industry and 25 control subjects. We found significant increases in LDH activities in the workers’ lung lavage, even in the absence of established silicosis (9 subjects). We looked at a similar observation in the sheep model of early silicosis, measured quartz content of lung lavage, and found significant correlation with LDH levels (R=0.64, p<0.001). All of the quartz particles in human and sheep lung lavage were in the alveolar macrophages. To test further the relationship of macrophage damage (cytotoxicity of quartz) we measured the release of LDH by sheep alveolar macrophage in 24 h cell culture under control conditions, exposure to inert dust, titanium, minusil-5 quartz, or aluminum-treated quartz. The LDH release was at control levels during titanium exposure and showed a significantly dose-related increase during quartz exposure. The latter cytotoxic effect was largely attenuated by aluminum treatment of quartz. These in vitro data agreed with previous reports. This study presents evidence of a cytotoxic effect of quartz inhalation in humans. The effect is related to the intensity of quartz retention in the lung macrophages; it is not a nonspecific dust exposure effect and can be attenuated by surface modification of the quartz.

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References

  1. Adamis Z, Timar M (1978) Studies on the effect of quartz, bentonite and coal dust mixtures on macrophages in vitro. Br J Exp Pathol 59:411–415

    PubMed  CAS  Google Scholar 

  2. Allison AC, Harington JS, Birbeck M (1966) An examination of the cytotoxic effects of silica on macrophages. J Exp Med 124:141–153

    Article  PubMed  CAS  Google Scholar 

  3. Beck EG (1976) Interaction between fibrous dust and cells in vitro. Ann Anat Pathol 12:227–236

    Google Scholar 

  4. Bégin R, Cantin A, Berthiaume Y, Boileau R, Bisson G, Lamoureux G, Rola-Pleszczynski M, Drapeau G, Massé S, Boctor M, Brault J, Péloquin S, Dalle D (1985) Clinical features to stage alveolitis in asbestos workers. Am J Indust Med 8: 521–536

    Article  Google Scholar 

  5. Bégin R, Martel M, Desmarais Y, Drapeau G, Boileau R, Rola-Pleszczynski M, Massé S (1986) Fibronectin and procollagen 3 levels in bronchoalveolar lavage of asbestos-exposed human subjects and sheep. Chest 89:237–243

    Article  PubMed  Google Scholar 

  6. Bégin R, Massé S, Rola-Pleszczynski M, Martel M, Desmarais Y, Geoffroy M, LeBouffant L, Daniel H, Martin J (1986) Aluminum lactate treatment alters the lung biological activity of quartz. Exp Lung Res 10:385–399

    Article  PubMed  Google Scholar 

  7. Bégin RO, Cantin AM, Boileau RD, Bisson GY (1987) Spectrum of alveolitis in quartz-exposed human subjects. Chest 92:1061–1067

    Article  PubMed  Google Scholar 

  8. Bégin R, Dufresne A, Cantin A, Possmayer F, Sébastien P (1989) Quartz exposure, retention and early silicosis in sheep. Exp Lung Res 15:409–428

    Article  PubMed  Google Scholar 

  9. Brody AR, Roe MW, Evans JN, Davis GS (1982) Deposition and translocation of inhaled silica in rats. Quantification of particle distribution, macrophage participation, and function. Lab Invest 47(6):533–542

    PubMed  CAS  Google Scholar 

  10. Cantin A, Crystal RG (1985) Oxidants, antioxidants and the pathogenesis of emphysema. Eur J Respir Dis 66 (suppl. 139):7

    Google Scholar 

  11. Cantin A, Dubois F, Bégin R (1988) Lung exposure to mineral dusts enhances the capacity of lung inflammatory cells to release superoxide. J Leuk Biol 43:299–303

    CAS  Google Scholar 

  12. Christman JW, Emerson RJ, Graham WGB, Davis GS (1985) Mineral dust and cell recovery from the bronchoalveolar lavage of healthy Vermont granite workers. Am Rev Respir Dis 132:393–399

    PubMed  CAS  Google Scholar 

  13. Davies R (1980) The effect of dusts on enzyme release from macrophages. In: Brown RL, Gormley IP, Chamberlain M, Davies R, eds. The in vitro effects of mineral dusts. Academic Press, London, pp 67–74

    Google Scholar 

  14. Davis GS (1986) Pathogenesis of silicosis: current concepts and hypotheses. Lung 164:139–154

    Article  PubMed  CAS  Google Scholar 

  15. Davis GS, Hemenway DR, Evans JN, Lapenas DJ, Brody AR (1981) Alveolar macrophage stimulation and population changes in silica-exposed rats. Chest 80S:8S-10S

    Article  Google Scholar 

  16. Dethloff LA, Gilmore LB, Gladen BC, George G, Chhabra RS, Hook GER (1986) Effects of silica on the composition of the pulmonary extracelluar lining. Toxicol Appl Pharmacol 84:66–83

    Article  PubMed  CAS  Google Scholar 

  17. Dubois F, Bégin R, Cantin A, Massé S, Martel M, Bilodeau G, Dufresne A, Perrault G, Sébastien P (1988) Aluminum inhalation reduces silicosis in a sheep model. Am Rev Respir Dis 137:1172–1179

    PubMed  CAS  Google Scholar 

  18. Emerit I, Keck M, Levy A, Feingold J, Michelson AM (1982) Activated oxygen species at the origin of chromosome breakage and sister-chromatid exchanges. Mutat Res 103:165

    Article  PubMed  CAS  Google Scholar 

  19. Fantone JC, Ward P (1984) Pathogenesis of the granulomatous lung diseases. Part III. Mechanisms of lung parenchymal injury. Am Rev Respir Dis 130:484–491

    CAS  Google Scholar 

  20. Fishman WH, Kato K, Anstiss CL, Green S (1967) Human serumβ-glucuronidase: its mèasurement and some of its properties. Clin Chim Acta 15:435–447

    Article  PubMed  CAS  Google Scholar 

  21. Gutteridge JMC, Westermarck T, Halliwell B (1985) Oxygen radical damage in biological systems. In: Free radicals and degenerative diseases Johnson JE, Walford R, Harman D, Miguel J, Eds. Alan R. Liss, New York, p 99

    Google Scholar 

  22. Hook GER (1978) Extracellular hydrolases of the lung. Biochemistry 17:520–528

    Article  PubMed  CAS  Google Scholar 

  23. ILO/UC International Classification of radiographs of pneumoconiosis (1980) No. 22 revised. Occupational safety and health series. International Labour Office, Geneva

  24. Killingsworth LM, Savory J (1972) Manual nephelometric methods for immunochemical determination of immunoglobulins IgG, IgA and IgM in human serum. Clin Chem 18:335–339

    PubMed  CAS  Google Scholar 

  25. Moores SR, Black A, Evans JC, Evans N, Holmes A, Morgan A (1981) The effect of quartz, administered by intratracheal instillation, on the rat lung. II. The short-term biochemical response. Environ Res 24:275–285

    Article  CAS  Google Scholar 

  26. Morgenstern S, Kessler G, Auerbach J, Flor RV, Klein B (1965) An automated p-nitrophenylphosphate serum alkaline phosphatase procedure for the autoanalyzer. Clin Chem 11:876–888

    PubMed  CAS  Google Scholar 

  27. Schuyler MR, Gaumer HR, Stankus RP, Kaimal J, Hoffmann E, Salvaggio JE (1980) Bronchoalveolar lavage in silicosis. Lung 157:95–102

    Article  PubMed  CAS  Google Scholar 

  28. Sjöstrand M, Rylander R (1984) Enzymes in lung lavage fluid after inhalation exposure to silica dust. Environ Res 33:307–311

    Article  PubMed  Google Scholar 

  29. Sjöstrand M, Rylander R (1987) Lysosomal enzyme activity and fibroblast stimulation of lavage from guinea pigs exposed to silica dust. Br J Exp Pathol 68:309–318

    PubMed  Google Scholar 

  30. Snedecor GW, Cochran WC (1967) Statistical methods. Iowa State University Press, Ames, IA

    Google Scholar 

  31. Sykes SE, Morgan A, Moores SR, Holmes A, Davison W (1983) Dose-dependent effects in the subacute response of the rat lung to quartz. I. The cellular response and the activity of lactate dehydrogenase in the airways. Exp Lung Res 5:229–243

    Article  PubMed  CAS  Google Scholar 

  32. Wacker W, Ulmer DD, Vallee BL (1956) Metalloenzymes and myocardial infarction: II. Malic and lactic dehydrogenase activities and zinc concentrations in serum. N Engl J Med 225:449–456

    Article  Google Scholar 

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Authors and Affiliations

  1. Unité de Recherche Pulmonaire, Université de Sherbrooke, Sherbrooke, Québec

    P. Larivée, A. Cantin & R. Bégin M.D.

  2. McGill University School of Occupational Health, Montréal, Canada

    A. Dufresne

Authors
  1. P. Larivée
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  2. A. Cantin
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  3. A. Dufresne
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  4. R. Bégin M.D.
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Larivée, P., Cantin, A., Dufresne, A. et al. Enzyme activities of lung lavage in silicosis. Lung 168, 151–158 (1990). https://doi.org/10.1007/BF02719686

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