Journal of Genetics

, Volume 83, Issue 3, pp 257–263 | Cite as

Cloning of a novel gene,Cymg1, related to family 2 cystatins and expressed at specific stages of mouse testis development

Research Article


We have cloned a novel gene,Cymg1 (GenBank accession number AY600990), from a mouse testis cDNA library.Cymg1 is located in 2G3 of mouse chromosome 2. The cDNA includes an open reading frame that encodes 141 amino acid residues. The encoded polypeptide has a cysteine protease inhibitor domain found in the family 2 cystatins but lacks critical consensus sites important for cysteine protease inhibition. These characteristics are seen in the proteins of the CRES subfamily of the family 2 cystatins which are expressed specifically in the reproductive tract. CYMG1 protein shows 44% identity with mouse CRES and 30% identity with mouse cystatin C. Northern blot analysis showed that theCymg1 gene was specifically expressed in adult mouse testis. RT-PCR also showed thatCymg1 was expressed in testis and spermatogonial cells.Cymg1 expression level varied in the different developmental stages of mouse testis, and were coincidental with spermatogenesis and sex maturation. These results indicate thatCymg1 may play important roles in mouse spermatogenesis and sex maturation


cystatins gene cloning testis spermatogenesis sex maturation 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Adams M. D., Kerlavage A. R., Fleischmann R. F., Bult C., Lee N., Kirkness al. 1995 Initial assessment of human gene diversity and expression patterns based upon 63 million nucleotides of cDNA sequences.Nature 377 (suppl.), 2–174.CrossRefGoogle Scholar
  2. Bourrouillou G., Dastugue N. and Colombies P. 1985 Chromosome studies in 952 infertile males with a sperm count below 10 million/ml.Hum. Genet. 71, 366–367.PubMedCrossRefGoogle Scholar
  3. Cornwall G. A. and Hann S. R. 1995 Transient appearance of CRES protein during spermatogenesis and caput epididymal sperm maturation.Mol. Reprod. Dev. 41, 37–46.PubMedCrossRefGoogle Scholar
  4. Cornwall G. A. and Hsia N. 2003 A new member of the CRES subgroup of family 2 cystatins.Mol. Cell. Endocrinol. 200, 1–8.PubMedCrossRefGoogle Scholar
  5. Cornwall G. A., Orgebin-Crist M. C. and Hann S. R. 1992 The CRES gene: a unique testis-regulated gene related to the cystatin family is highly restricted in its expression to the proximal region of the mouse epididymis.Mol. Endocrinol. 6, 1653–1664.PubMedCrossRefGoogle Scholar
  6. Gill R. W. and Sanseau P. 2000 Rapid in silico cloning of genes using expression sequence tags (ESTs).Biotechnol. Annu. Rev. 5, 25–44.PubMedCrossRefGoogle Scholar
  7. Luk J. M., Mok B. W., Shum C. K., Yeung W. S., Tam P. C., Tse J. al. 2003 Identification of novel genes expressed during spermatogenesis in stage-synchronized rat testes by differential display.Biochem. Biophys. Res. Commun. 307, 782–790.PubMedCrossRefGoogle Scholar
  8. Pruitt K. D., Katz K. S., Sicotte H. and Maglott D. R. 2000 Introducing Ref Seq and Locus Lingk: Curated human genome resources at the NCBI.Trends Genet. 16, 44–47.PubMedCrossRefGoogle Scholar
  9. Qian J., Zhang X. M., Li X. L., Wang J. R., Li W. F., Wang al. 2002 Identification of digital differential expression 263 patterns of a novel human gene (UBAP1) by an expressed sequence tag strategy.Ai Zheng 21, 225–228.PubMedGoogle Scholar
  10. Sato M., Murao K., Mizobuchi M. and Takahara J. 1993 Quantitative and sensitive Northern blot hybridization using PCR-generated DNA probes labeled with digoxigenin by nick translation.Biotechniques 15, 880–882.PubMedGoogle Scholar
  11. Stephen F. M., Barry O. D., Simon J. F., Hugh R. B., Guenole S., Peter P. D. 2003 Digital extractor: analysis of digital differential display output.Bioinformatics 12, 1594–1595.Google Scholar
  12. Sutton H. G., Fusco A. and Cornwall G. A. 1999 Cystatin-related epididymal spermatogenic protein colocalizes with luteinizing hormone-beta protein in mouse anterior pituitary gonadotropes.Endocrinology 140, 2721–2732.PubMedCrossRefGoogle Scholar
  13. Syntin P. and Cornwall G. A. 1999 Immunolocalization of CRES (cystatin-related epididymal spermatogenic) protein in the acrosomes of mouse spermatozoa.Biol. Reprod. 60, 1542–1552.PubMedCrossRefGoogle Scholar
  14. Turk V. and Bode W. 1991 The cystatins: protein inhibitors of cysteine proteinases.FEBS Lett. 285, 213–219.PubMedCrossRefGoogle Scholar
  15. Vogt P. H., Edelmann A., Kirsch S., Henegariu O., Hirschmann P., Kiesewetter al. 1996 Human Y chromosome azoospermia factors (AZF) mapped to different subregions in Yq11.Hum. Mol. Genet. 5, 933–943.PubMedCrossRefGoogle Scholar
  16. Xing X. W., Li L. Y., Fu J. J., Zhu W. B., Liu G., Liu S. al. 2003 Cloning of cDNA ofTSARG4, a human spermatogenesis related gene.Acta Biochim. Biophys. Sin. 35, 283–288.PubMedGoogle Scholar

Copyright information

© Indian Academy of Sciences 2004

Authors and Affiliations

  1. 1.Human Reproductive and Stem Cell Engineering InstituteCentral South UniversityChangshaPeople’s Republic of China

Personalised recommendations