Journal of Chemical Sciences

, Volume 115, Issue 2, pp 123–128 | Cite as

Kinetics and mechanism of protection of thymine from sulphate radical anion under anoxic conditions



The rates of photooxidation of thymine in presence of peroxydisulphate (PDS) have been determined by measuring the absorbance of thymine at 264 nm spectrophotometrically. The rates and the quantum yields (φ) of oxidation of thymine by sulphate radical anion have been determined in the presence of different concentrations of caffeic acid. Increase in [caffeic acid] is found to decrease the rate of oxidation of thymine suggesting that caffeic acid acts as an efficient scavenger of SO 4 •- and protects thymine from it. Sulphate radical anion competes for thymine as well as for caffeic acid. The rate constant of sulphate radical anion with caffeic acid has been calculated to be 1.24 x 1010 dm3 mol-1s-1. The quantum yields of photooxidation of thymine have been calculated from the rates of oxidation of thymine and the light intensity absorbed by PDS at 254 nm, the wavelength at which PDS is activated to sulphate radical anion. From the results of experimentally determined quantum yields (φexpt1) and the quantum yields calculated (φcl) assuming caffeic acid acting only as a scavenger of SO 4 •- radicals show that φexpt1 values are lower than φcl values. The φ ’ values, which are experimentally found quantum yield values at each caffeic acid concentration and corrected for SO 4 •- scavenging by caffeic acid, are also found to be greater than φexpt1 values. These observations suggest that the thymine radicals are repaired by caffeic acid in addition to scavenging of sulphate radical anions.


Oxidation of caffeic acid repair of thymine by caffeic acid oxidations by sulphate radical anion 


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  1. 1.
    von Sonntag C 1987The chemical basis of radiation biology (London: Taylor & Francis)Google Scholar
  2. 2.
    Hutchinson F 1985Progr. Nucleic Acid Res. Mol. Biol. 32 115CrossRefGoogle Scholar
  3. 3.
    Adinarayana M, Bothe E and Shulte-Frohlinde D 1988Int. J. Radiat. Biol. 54 723CrossRefGoogle Scholar
  4. 4.
    Lemaire D G E, Bothe E and Sculte-Frohlinde D 1984Int. J. Radiat. Biol. 45 351CrossRefGoogle Scholar
  5. 5.
    Bansal K M and Fessenden R W 1978Radiat. Res. 75 497CrossRefGoogle Scholar
  6. 6.
    Ravi Kumar M and Adinarayana M 2001Int. J. Chem. Kinet. 33 271CrossRefGoogle Scholar
  7. 7.
    Kapoor S, Sharma P D and Gupta Y K 1975Talanta 22 765CrossRefGoogle Scholar
  8. 8.
    Ravi Kumar M, Thirupathi Rao M and Adinarayana M 1998Indian J. Chem. A37 346Google Scholar
  9. 9.
    Deeble D J, Schuchmann M N, Steenken S and von Sonntag C 1990J Phys. Chem. 94 8186CrossRefGoogle Scholar
  10. 10.
    Akhalaq M S, Al-Baghdad S and von Sonntag C 1987Carbohydrate Res. 164 71CrossRefGoogle Scholar

Copyright information

© Indian Academy of Sciences 2003

Authors and Affiliations

  1. 1.Department of ChemistryOsmania UniversityHyderabadIndia

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