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Journal of Biosciences

, Volume 9, Issue 1–2, pp 23–33 | Cite as

Effects of protein deficiency on selective elicitation of lysosomal enzymes in rat peritoneal macrophages

  • U. R. Iyengar
  • U. K. Vakil
Article
  • 27 Downloads

Abstract

Young albino rats were fedad libitum 4, 8 or 20 % (control) protein diet for 1–4 weeks. Total activities of some of the lysosomal enzymes, namely, acid phosphatase, aryl sulphatase, Β-glucuronidase and cathepsin D, were determined in resident and protease-peptone elicited peritoneal macrophages. Total cell number, protein content and the lysosomal enzyme activities were increased significantly in protease-peptone elicited macrophages; though at a lower rate in 4 % protein-fed group compared to control ones. However, the rate of induction of the tested hydrolases was selective and their response to the stimulant varied widely. Similarly, response of each enzyme to low protein diet also varied. Thus, at 4 weeks, cathepsin D and Β-glucuronidase activities, expressed per total number of elicited macrophages were reduced by 45 and 60 %, respectively, in 4 % protein-fed animals. These results indicate that the metabolic events related to lysosomal function in macrophages, are affected by dietary restriction of proteins

Keywords

Rat protein deficiency peritoneal macrophages elicitation lysosomal enzymes 

Abbreviations

(4-MU)

4-Methylumbelliferone

PBS

phosphate buffer saline solution

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Copyright information

© Indian Academy of Sciences 1985

Authors and Affiliations

  • U. R. Iyengar
    • 1
  • U. K. Vakil
    • 1
  1. 1.Biochemistry and Food Technology DivisionBhabha Atomic Research CentreBombayIndia

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