Journal of Biosciences

, Volume 9, Issue 1–2, pp 23–33 | Cite as

Effects of protein deficiency on selective elicitation of lysosomal enzymes in rat peritoneal macrophages

  • U. R. Iyengar
  • U. K. Vakil


Young albino rats were fedad libitum 4, 8 or 20 % (control) protein diet for 1–4 weeks. Total activities of some of the lysosomal enzymes, namely, acid phosphatase, aryl sulphatase, Β-glucuronidase and cathepsin D, were determined in resident and protease-peptone elicited peritoneal macrophages. Total cell number, protein content and the lysosomal enzyme activities were increased significantly in protease-peptone elicited macrophages; though at a lower rate in 4 % protein-fed group compared to control ones. However, the rate of induction of the tested hydrolases was selective and their response to the stimulant varied widely. Similarly, response of each enzyme to low protein diet also varied. Thus, at 4 weeks, cathepsin D and Β-glucuronidase activities, expressed per total number of elicited macrophages were reduced by 45 and 60 %, respectively, in 4 % protein-fed animals. These results indicate that the metabolic events related to lysosomal function in macrophages, are affected by dietary restriction of proteins


Rat protein deficiency peritoneal macrophages elicitation lysosomal enzymes 





phosphate buffer saline solution


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Adhikari, H. R., Vakil, U. K. and Sreenivasan, A. (1972)Indian J. Biochem. Biophys.,9, 89.PubMedGoogle Scholar
  2. Brown, J. A. and Swank, R. T. (1983)J. Biol. Chem.,258, 15323.PubMedGoogle Scholar
  3. Bursuker, I. and Goldman, R. (1983)J. Reticuloendothel. Soc.,33, 207.PubMedGoogle Scholar
  4. Chandra, R. K. (1980) inImmunology of Nutrition Disorders, Yearbook, Medical Publishers, Inc., Chicago, p. 16.Google Scholar
  5. Chandra, R. K. and Newberne, P. M. (1977) inNutrition, Immunity and Infection: Mechanism of infections, (New York: Plenum Publishing Corporation,) p. 45.Google Scholar
  6. Creek, K. E. and Sly, W. S. (1983)Biochemistry,214, 353.Google Scholar
  7. Davis, P., Page, R. C. and Allison, A. C. (1974)J. Exp. Med.,139, 1262.CrossRefGoogle Scholar
  8. Douglas, S. D. and Schopfer, P. (1974)Clin. Exp. Immunol.,17, 121.PubMedGoogle Scholar
  9. Edward, L. P. and Stanton, G. A. (1975)J. Exp. Med.,141, 1030.CrossRefGoogle Scholar
  10. Goodrum, K. J. and Spitznagel, J. K. (1982)J. Reticuloendothel. Soc.,31, 339.PubMedGoogle Scholar
  11. Hambor, J. E., Fleck, L. and Stevenson, J. R. (1983)Cell Immunol.,81, 306.PubMedCrossRefGoogle Scholar
  12. Hamm, M. W. and Winick, M. (1984)J. Nutr.,144, 511.Google Scholar
  13. Hasilik, A. and Newfeld, E. (1980)J. Biol. Chem.,255, 4946.PubMedGoogle Scholar
  14. Hirschhorn, K. and Hirschhorn, R. (1965)Lancet,1, 1046.CrossRefGoogle Scholar
  15. Iyengar, U. R. and Vakil, U. K. (1985)J. Biosci.,7, 15.CrossRefGoogle Scholar
  16. Mead, J. A. R., Smith, J. N. and Williams, R. T. (1955)Biochem. J.,61, 569.PubMedGoogle Scholar
  17. Miller, G. L. (1959)Anal. Chem.,28, 350.Google Scholar
  18. Morland, B. and Morland, J. (1978)J. Reticuloendothel. Soc.,23, 464.Google Scholar
  19. Munoz, E., Marcos, A. and Unzaga, M. T. (1981)J. Nutr.,111, 2133.PubMedGoogle Scholar
  20. Ohnishi, O. (1984)Clin. Exp. Immunol.,58, 206.PubMedGoogle Scholar
  21. Peters, T. J., Muller, H. and DeDuve, C. (1972)J. Exp. Med.,136, 1117.PubMedCrossRefGoogle Scholar
  22. Roth, J. S. and Locty, T. (1971)Anal. Biochem.,42, 214.PubMedCrossRefGoogle Scholar
  23. Snedecor, G. M. and Cochran, W. G. (1967) inStatistical Methods, (Iowa: The Iowa University Press) p. 91.Google Scholar
  24. Stahl, P. and Touster, O. (1971)J. Biol. Chem.,246, 5398.PubMedGoogle Scholar
  25. Takano, Y., Imai, K., Tanaka, A., Fujimori, K., Yamada, M. and Yamamoto, K. (1983)CellMol.Biol.,29, 373.Google Scholar
  26. Tomino, I. (1979)Metab. Dis.,16, 129.Google Scholar
  27. Umana, R. (1967)Can. J. Biochem.,45, 1353.PubMedCrossRefGoogle Scholar
  28. Unanue, E. R. (1978)Immunol. Rev.,40, 227.PubMedCrossRefGoogle Scholar
  29. Weeks, B. A. and Kavas, A. F. (1979)J. Reticuloendothel. Soc.,26, 501.PubMedGoogle Scholar

Copyright information

© Indian Academy of Sciences 1985

Authors and Affiliations

  • U. R. Iyengar
    • 1
  • U. K. Vakil
    • 1
  1. 1.Biochemistry and Food Technology DivisionBhabha Atomic Research CentreBombayIndia

Personalised recommendations