Journal of Biosciences

, Volume 14, Issue 3, pp 261–268 | Cite as

Purification and some properties of buffalo spleen cathepsin B

  • Sarfraz Ahmad
  • Sudhir K. Agarwal
  • M. Yahiya Khan


Purification of cathepsin B from buffalo-spleen, a hitherto unstudied system has been achieved by a simple procedure developed by incorporating suitable modifications in the existing methods for isolation of the enzyme from other sources. The purified enzyme has a molecular weight of 25 KDa and its Stokes radius was found to be 2·24 nm. Effects of several reducing agents, urea and thiol-protease inhibitors such as leupeptin and antipain, have been studied and the data unequivocally support the contention that the buffalo-enzyme is similar to cathepsin B from other tissues with respect to these properties.


Lysosomal proteases cathepsin B buffalo-spleen hydrodynamic properties 

Abbreviations used








sodium dodecyl sulphate


bovine serum albumin


5,5′-dithio-bis-(2-nitrobenzoic acid)


Polyacrylamide gel electrophoresis


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Copyright information

© Indian Academy of Sciences 1989

Authors and Affiliations

  • Sarfraz Ahmad
    • 1
  • Sudhir K. Agarwal
    • 1
  • M. Yahiya Khan
    • 1
  1. 1.Department of Biochemistry, School of Life SciencesNorth-Eastern Hill UniversityShillongIndia

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