Journal of Biosciences

, Volume 10, Issue 3, pp 311–317 | Cite as

Hydrogen peroxide formation and lipid peroxidation in rat uterus—effect of hormones and vitamin E

  • Shyamali Mukherjee
  • Mihir Nag
  • Tultul Nayyar
  • Indrani Maitra
  • Parul Chakrabarti
  • Pramod R. Dasgupta


The effect of estradiol-17β and progesterone given separately as well as in combination on the rate of hydrogen peroxide formation and lipid peroxidation in the uteri of ovariectomized rats was studied. Estradiol in 3μg dose per day per animal elicited maximum stimulatory response and progesterone (100μg), on the other hand, was without any such effect. However, progesterone given along with estradiol completely prevented the effect due to the latter. In the same way, vitamin E, a well known antioxidant was found to be extremelv effective in protecting the uterus from the highly peroxidative action of estradiol-17β.


H2O2-formation lipid peroxidation free radicals malonaldehyde-thiobarbituric reaction estradiol-17β progesterone vitamin E (dl-α-tocopherol) 

Abbreviations used


Unsaturated fatty acid




sodium dodecyl sulphate


thiobarbituric acid


intrauterine contraception devices


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Copyright information

© Indian Academy of Sciences 1986

Authors and Affiliations

  • Shyamali Mukherjee
    • 1
  • Mihir Nag
    • 1
  • Tultul Nayyar
    • 1
  • Indrani Maitra
    • 1
  • Parul Chakrabarti
    • 1
  • Pramod R. Dasgupta
    • 1
  1. 1.Department of ChemistryBose InstituteCalcuttaIndia

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