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Journal of Biosciences

, Volume 5, Issue 3, pp 209–217 | Cite as

Effect of denaturants on the 11S protein of guar seed (Cyamopsis tetragonoloba)

  • J. P. Nath
  • M. S. Narasinga Rao
Article

Abstract

The effect of sodium dodecyl sulphate, urea or guanidinium hydrochloride on the sedimentation velocity, viscosity, ultra violet spectra and fluorescence spectra of the 11S protein of guar seed has been determined. Sodium dodecyl sulphate dissociates the protein directly to the 2S protein, whereas urea or guanidinium hydrochloride produces an intermediate 7S protein. These reagents denature the protein also. Both the dissociative and the denaturation effect follow the order, sodium dodecyl sulphate > guanidinium hydrochloride > urea when the concentration are expressed as mols per litre. The denatured states in the three cases probably differ.

Keywords

Denaturation guar seed 11S protein 

Abbreviations used

SDS

Sodium dodecyl sulphate

GdmCl

guanidinium hydrochloride

PAGE

polyacrylamide gel electrophoresis

DEAE

diethyl amino ethyl

Mr

molecular weight

RTIC

rotor temperature indicator and control

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Copyright information

© Indian Academy of Sciences 1983

Authors and Affiliations

  • J. P. Nath
    • 1
  • M. S. Narasinga Rao
    • 1
  1. 1.Protein technology DisciplineCentral Food Technological Research InstituteMysore

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