Effect of denaturants on the 11S protein of guar seed (Cyamopsis tetragonoloba)
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The effect of sodium dodecyl sulphate, urea or guanidinium hydrochloride on the sedimentation velocity, viscosity, ultra violet spectra and fluorescence spectra of the 11S protein of guar seed has been determined. Sodium dodecyl sulphate dissociates the protein directly to the 2S protein, whereas urea or guanidinium hydrochloride produces an intermediate 7S protein. These reagents denature the protein also. Both the dissociative and the denaturation effect follow the order, sodium dodecyl sulphate > guanidinium hydrochloride > urea when the concentration are expressed as mols per litre. The denatured states in the three cases probably differ.
KeywordsDenaturation guar seed 11S protein
Sodium dodecyl sulphate
polyacrylamide gel electrophoresis
diethyl amino ethyl
rotor temperature indicator and control
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