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Resorbable ciprofloxacin/polyglycol acid carrier in the local therapy of chronic osteitis

  • S. Winckler
  • J. P. Overbeck
  • R. Meffert
  • P. Törmälä
  • H. -U. Spiegel
European Bone And Joint Infection Society Meeting, München, Germany — October 7–9, 1993

Summary

Polyglycolic test cylinders (PGA) of different sizes and with a different amount of Ciprofloxacin were synthezised and tested in vitro. The monofil 3,2×5 mm sized cylinders containing 3 mg of Ciprofloxacin showed the best release of the antibiotic up to half a year (measured by HPLC). In vivo setting: in the right femur of 66 rabbits osteomyelitis was induced by injection of 3×106 cfu of staph, aureus after the bone marrow had been sclerosed with sodium morrhuate 5%. Two weeks later test cylinders were implanted into the femoral shaft of 30 animals. Thirty-six animals served as controls. Criteria evaluated: weight change, laboratory findings, microbiological, radiological and histopathological parameters. The concentration of Ciprofloxacin was measured in serum, urine and wound secretion by HPLC. All animals developed osteomyelitis. Swabs from the marrow of all treated animals were sterile. The histopathological findings in the control group showed the mild or strong chronic-persistent form of osteomyelitis. In the therapy group even after 2 weeks we mainly found the the chronic-fibrous form. The test-cylinders were well tolerated in the marrow. Levels of the drug were measured in serum, urine and wound secretion.

Key words

Bone infection Experimental study Polyglycol acid carrier 

Code Méary

0825.5 1103.0 

Supports résorbables de ciprofloxacine-polyglycol acide dans le traitement local des ostéites chroniques

Résumé

Des cylindres test d’acide polyglicolique (PGA) de différentes tailles et avec un gradient variable de ciprofloxacine furent synthétisés et testés in vitro. Le cylindre monofil (taille 3,2×5 mm) contenant 3 mg de ciprofloxacine a montré le meilleur relargage d’antibiotiques jusqu’à 6 mois (mesuré par HPLC). Dans l’étude in vivo, une ostéomyélite fut induite dans le fémur droit de 66 lapins par injection de 3×106 cfu de staphylocoque aureus après destruction médullaire par du sodium morrhuate 5%. Deux semaines après les cylindres test furent implantés dans le fémur de 30 animaux. Les 36 autres animaux servant de groupe témoins. Les critères d’evaluation furent. : la courbe de poids, les analyses biologiques, microbiologiques, radiologiques et histopathologiques. La concentration de ciprofloxacine fut mesurée dans le sérum, les urines et la plaie par HPLC. Tous les animaux développèrent une ostéomyélite. Le prélèvement de la moëlle de tous les animaux traités fut stérile. Les études histopathologiques du groupe témoin montrèrent une ostéomyélite persistante plus ou moins importante. Dans le groupe traité après 2 semaines les auteurs trouvent une forme fibreuse chronique. Les cylindres tests furent bien tolérés dans la moëlle.

Mots-clés

Ostéomyélite Etude expérimentale Support d’acide polyglicolique 

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • S. Winckler
    • 1
  • J. P. Overbeck
    • 1
  • R. Meffert
    • 1
  • P. Törmälä
    • 3
  • H. -U. Spiegel
    • 2
  1. 1.Department of Trauma- and Hand SurgeryWestfälische Wilhelms-UniversitätMünsterGermany
  2. 2.Department of General Surgery (Surg. Research)Westfälische Wilhelms-UniversitätMünsterGermany
  3. 3.Institute of Plastics TechnologyTechnican University of TampereFinland

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