, Volume 169, Issue 1, pp 247–254 | Cite as

Effect of IgE-stimulated alveolar macrophages on tracheal epithelial bioelectric properties in dogs

  • J. Tamaoki
  • A. Chiyotani
  • K. Isono
  • N. Sakai
  • T. Kanemura
  • T. Takizawa


To investigate a possible interaction between pulmonary alveolar macrophages (AMs) and airway epithelial cells in patients with allergic conditions, we studied the effect of AMs on bioelectric properties of canine tracheal epithelium under short-circuited conditions in vitro. Mucosal addition of the supernatants from AMs stimulated with monoclonal antidinitrophenyl (DNP) IgE antibody and DNP-human serum albumin (DNP-HSA) increased short-circuit current (Isc) of cultured epithelium in a dose-dependent manner. The maximal increase from the baseline value and the EC50 were 10.2±2.0µA/cm2 (mean ± SE, p<0.01) and 3×105 AMs/ml, respectively. This effect was accompanied by the release of prostaglandin E2 and F from AMs. In contrast, AMs incubated with anti-DNP IgE antibody alone or DNP-HSA alone had no effect. The AM-induced increase in Isc was attenuated by diphenylamine-2-carboxylate and Cl-free medium but not by amiloride. Pretreatment of AMs with indomethacin or piroxicam inhibited the effect of AMs on epithelial Isc. These results suggest that AMs may stimulate Cl secretion across the airway mucosa through an IgE-dependent release of prostaglandins.

Key words

Macrophage Epithelium Ion transport Allergy Asthma 


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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • J. Tamaoki
    • 1
  • A. Chiyotani
    • 1
  • K. Isono
    • 1
  • N. Sakai
    • 1
  • T. Kanemura
    • 1
  • T. Takizawa
    • 1
  1. 1.Pulmonary Division, First Department of MedicineTokyo Women’s Medical CollegeTokyoJapan

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