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Lung

, Volume 169, Issue 1, pp 247–254 | Cite as

Effect of IgE-stimulated alveolar macrophages on tracheal epithelial bioelectric properties in dogs

  • J. Tamaoki
  • A. Chiyotani
  • K. Isono
  • N. Sakai
  • T. Kanemura
  • T. Takizawa
Article
  • 22 Downloads

Abstract

To investigate a possible interaction between pulmonary alveolar macrophages (AMs) and airway epithelial cells in patients with allergic conditions, we studied the effect of AMs on bioelectric properties of canine tracheal epithelium under short-circuited conditions in vitro. Mucosal addition of the supernatants from AMs stimulated with monoclonal antidinitrophenyl (DNP) IgE antibody and DNP-human serum albumin (DNP-HSA) increased short-circuit current (Isc) of cultured epithelium in a dose-dependent manner. The maximal increase from the baseline value and the EC50 were 10.2±2.0µA/cm2 (mean ± SE, p<0.01) and 3×105 AMs/ml, respectively. This effect was accompanied by the release of prostaglandin E2 and F from AMs. In contrast, AMs incubated with anti-DNP IgE antibody alone or DNP-HSA alone had no effect. The AM-induced increase in Isc was attenuated by diphenylamine-2-carboxylate and Cl-free medium but not by amiloride. Pretreatment of AMs with indomethacin or piroxicam inhibited the effect of AMs on epithelial Isc. These results suggest that AMs may stimulate Cl secretion across the airway mucosa through an IgE-dependent release of prostaglandins.

Key words

Macrophage Epithelium Ion transport Allergy Asthma 

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References

  1. 1.
    Al-Bazzaz FJ, Cheng E (1979) Effect of catecholamines on ion transport in dog tracheal epithelium. Am J Physiol 239:C112-C117Google Scholar
  2. 2.
    Al-Bazzaz FJ, Yadava VP, Westenfelder C (1981) Modification of Na and Cl transport in canine tracheal mucosa by prostaglandins. Am J Physiol 234:F101-F105Google Scholar
  3. 3.
    Al-Bazzaz FJ, Zevin R (1984) Ion transport and metabolic effects of amiloride in canine tracheal mucosa. Lung 162:357–367PubMedCrossRefGoogle Scholar
  4. 4.
    Balter MS, Eschenbacher WL, Peters-Golden M (1988) Arachidonic acid metabolism in cultured alveolar macrophages from normal, atopic, and asthmatic subjects. Am Rev Respir Dis 138:1134–1142PubMedGoogle Scholar
  5. 5.
    Boucher RC, Larsen EH (1988) Comparison of ion transport by cultured secretory and absorptive epithelia. Am J Physiol 254:C535-C547.PubMedGoogle Scholar
  6. 6.
    DiStefano A, Wittner M, Schlatter E, Lang FJ, Englert H, Greger R (1986) Diphenylamine-2-carboxylate, a blocker of the Cl conductive pathway in Cl-transporting epithelia. Pfluegers Arch 405:S95-S100CrossRefGoogle Scholar
  7. 7.
    Eschenbacher WL, Gravelyn TR (1987) A technique for isolated airway segment lavage. Chest 92:105–109PubMedGoogle Scholar
  8. 8.
    Fels AOS, Cohn ZA (1986) The alveolar macrophage. J Appl Physiol 60:353–369PubMedGoogle Scholar
  9. 9.
    Jose P, Niederhauser U, Piper PJ, Robinson C, Smith AP (1976) Degradation of prostaglandin F in the human pulmonary circulation. Thorax 31:713–719PubMedCrossRefGoogle Scholar
  10. 10.
    Joseph M, Tonnel AB, Torpier G, Capron A, Arnoux B, Benveniste J (1983) Involvement of immunoglobulin E in the secretory process of alveolar macrophages from asthmatic patients. J Clin Invest 72:221–230Google Scholar
  11. 11.
    Leikauf GD, Ueki IF, Widdicombe JH, Nadel JA (1986) Alteration of chloride secretion across canine tracheal epithelium by lipoxygenase products of arachidonic acid. Am J Physiol 250:F47-F53PubMedGoogle Scholar
  12. 12.
    Melewicz FM, Kline LE, Cohen AB, Spiegelberg HL (1982) Characterization of IgE receptor for IgE on human alveolar macrophages. Clin Exp Immunol 49:364–370PubMedGoogle Scholar
  13. 13.
    Nadel JA, Wiiddicombe JH, Peafield AC (1985) Regulation of airway secretions, ion transport, and water movement. In: Fishman AP (eds) Handbook of physiology, section 3. American Physiological Society, Bethesda, MD, pp 419–445Google Scholar
  14. 14.
    Nathan CF (1987) Secretory products of macrophages. J Clin Invest 79:319–326PubMedGoogle Scholar
  15. 15.
    Rankin JA, Hitchcock M, Merrill W, Bach MK, Brashler JR, Askenase PW (1982) IgE-dependent release of leukotriene C4 from alveolar macrophages. Nature 297:329–331PubMedCrossRefGoogle Scholar
  16. 16.
    Schulz R, Seeger W (1986) Release of leukotrienes into the perfusate of calcium-ionophore stimulated rabbit lungs: influence of 5-lipoxygenase inhibitors. Biochem Pharmacol 35:183–193PubMedCrossRefGoogle Scholar
  17. 17.
    Shaw SE, Anderson NV (1984) Isolation and functional analysis of normal canine blood monocytes and resident alveolar macrophages. Am J Vet Res 45:87–90PubMedGoogle Scholar
  18. 18.
    Smith PL, Welsh MJ, Stoff JS, Frizzell RA (1982) Chloride secretion by canine tracheal epithelium. I. Role of intracellular cAMP levels. J Membrane Biol 70:217–226CrossRefGoogle Scholar
  19. 19.
    Spiegelberg HL (1984) Structure and function of Fc receptors for IgE on lymphocytes, monocytes, and macrophages. Adv Immunol 35:61–88PubMedCrossRefGoogle Scholar
  20. 20.
    Tamaoki J, Sekizawa K, Ueki IF, Graf PD, Nadel JA, Bigby TD (1987) Effect of macrophage stimulation on parasympathetic airway contraction in dogs. Eur J Pharmacol 138:421–425PubMedCrossRefGoogle Scholar
  21. 21.
    Welsh MJ, Widdicombe JH, Nadel JA (1980) Fluid transport across the canine tracheal epithelium. J Appl Physiol 49:905–909PubMedGoogle Scholar
  22. 22.
    Widdicombe JH, Welsh MJ (1980) Anion selectivity of the chloride transport process in dog tracheal epithelium. Am J Physiol 239:C112–117PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • J. Tamaoki
    • 1
  • A. Chiyotani
    • 1
  • K. Isono
    • 1
  • N. Sakai
    • 1
  • T. Kanemura
    • 1
  • T. Takizawa
    • 1
  1. 1.Pulmonary Division, First Department of MedicineTokyo Women’s Medical CollegeTokyoJapan

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