, Volume 164, Issue 1, pp 259–268 | Cite as

Acute effects of oleic acid-induced lung injury in baboons

  • James F. Collins
  • Ruben de los Santos
  • W. G. Johanson


Oleic acid induces lung injury in the baboon. Within 90 min of intravenous oleic acid injection, there are significant increases relative to baseline in the bronchoalveolar lavage fluid (BALF) contents of total protein, lactic dehydrogenase, hexosaminidase, acid protease, and angiotensin-converting enzyme, indicating increased alveolar permeability. When BALF is chromatographed on Sephadex G-200, the elution profiles of hexosaminidase, acid protease, and angiotensin-converting enzyme in BALF are similar to those from normal plasma. SDS-polyacrylamide gel electrophoresis of BALF proteins indicates a similar molecular weight distribution in baseline and post-oleic-acid BALF. No evidence of serine proteases active in the inflammatory process, and present in many cases of human adult respiratory failure, is found either by incubating BALF with hemoglobin and elastin substrates or by labeling BALF with3H-diisopropylfluorophosphate. However, there is a significant increase in α1-antiprotease activity in BALF relative to the baseline value. The activities in plasma of several acute phase reactants including antiproteases, fibronectin, and angiotensin-converting enzyme do not change in this acute phase of the lung injury. The data indicate that many biochemical markers used to indicate acute respiratory failure in humans do not change acutely in the expected fashion in this animal model of the disease.

Key words

Lung injury Papio-oleic acid 


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  1. 1.
    Beatty K, Robertie P, Senior RM, Travis J (1982) Determination of oxidized alpha-1-proteinase inhibitor in serum. J Lab Clin Med 100:186–192PubMedGoogle Scholar
  2. 2.
    Bedrossian CWM, Woo J, Miller WC, Cannon DC (1978) Decreased angiotensin converting enzyme in the adult respiratory distress syndrome. Am J Clin Pathol 70:244–247PubMedGoogle Scholar
  3. 3.
    Bell RC, Coalson JJ, Smith JD, Johanson WG Jr (1983) Multiple organ failure and infection in adult respiratory distress syndrome. Ann Intern Med 99:293–298PubMedGoogle Scholar
  4. 4.
    Campbell GD, Coalson JJ, Johanson WG Jr (1984) The effect of bacterial superinfection on lung function after diffuse alveolar damage. Am Rev Respir Dis 129:974–978PubMedGoogle Scholar
  5. 5.
    Collins JF, Smith JD, Coalson JJ, Johanson WG Jr (1984) Variability in lung collagen amounts after prolonged support of acute respiratory failure. Chest 85:641–646PubMedGoogle Scholar
  6. 6.
    Dubois RM, Townsend PJ, Cole PJ (1980) Alveolar macrophage lysosomal enzyme and C3b receptors in crytogenic fibrosing alveolitis. Clin Exp Immunol 40:60–65Google Scholar
  7. 7.
    Erlanger BF, Kokowsky N, Cohen W (1961) The preparation and properties of two new chromogenic substrates of trypsin. Arch Biochem Biophys 95:271–278PubMedCrossRefGoogle Scholar
  8. 8.
    Fine R, Campbell ME, Collins JF (1984) Elastolytic activity of peripheral blood monocytes. Connective Tissue Res 13:27–35Google Scholar
  9. 9.
    Fine R, Collins JF (1982) The effect of theophylline on macrophage elastase secretion. Connective Tissue Res 9:195–199CrossRefGoogle Scholar
  10. 10.
    Fowler AA, Hamman RF, Good JT, Benson KN, Baird M, Eberle D, Petty TL, Hyers TM (1983) The adult respiratory distress syndrome: risk with common predispositions. Ann Intern Med 98:593–597PubMedGoogle Scholar
  11. 11.
    Fowler AA, Hamman RF, Zerbe GO, Benson KN, Hyers TM (1985) Adult respiratory distress syndrome: prognosis after onset. Am Rev Respir Dis 132:472–478PubMedGoogle Scholar
  12. 12.
    Fowler AA, Walchak S, Giclas PC, Henson PH, Hyers TM (1982) Characterization of antiproteinase activity in the adult respiratory distress syndrome. Chest 81:50S-51SCrossRefGoogle Scholar
  13. 13.
    Gadek JE, Kelman JA, Fells G, Weinberger SE, Horowitz AL, Reynolds HY, Fulmer JD, Crystal RG (1979) Collagenase in the lower respiratory tract of patients with idiopathic pulmonary fibrosis. N Engl J Med 301:737–742PubMedCrossRefGoogle Scholar
  14. 14.
    Grossman J, Will L, Hahn N, Exten R, Garber C, Mosher D (1983) Plasma fibronectin in critical illness. Am Rev Respir Dis 127:95 (abstr)Google Scholar
  15. 15.
    Horowitz AL, Kelman JA, Crystal RG (1976) Activation of alveolar macrophage collagenase by a neutral protease secreted by the same cell. Nature (London) 264:772–774CrossRefGoogle Scholar
  16. 16.
    Johanson WG Jr, Holcomb JR, Coalson JJ (1982) Experimental diffuse alveolar damage in baboons. Am Rev Respir Dis 126:142–151PubMedGoogle Scholar
  17. 17.
    Labrousse J, Couland JM, Jacqueson A, Rapin J, Beyne P, Salmona JF, Tenaillon A, Lissac J (1982) Plasma fibronectin concentration in acute respiratory failure. Am Rev Respir Dis 125:132 (abstr)Google Scholar
  18. 18.
    Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680–685CrossRefGoogle Scholar
  19. 19.
    Lee CT, Fein AM, Lippmann M, Holtzman H, Kimbel P, Weinbaum G (1981) Elastolytic activity in pulmonary lavage fluid from patients with adult respiratory distress syndrome. N Engl J Med 304:192–196PubMedCrossRefGoogle Scholar
  20. 20.
    Lieberman J (1975) Elevation of serum angiotensin-converting enzyme in sarcoidosis. 59:365–372Google Scholar
  21. 21.
    Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folinphenol reagent. J Biol Chem 193:265–275PubMedGoogle Scholar
  22. 22.
    Maunder RJ, Harlan JM, Pepe PE, Holtman Revs D, Hudson LD, Carrico CJ (1983) Fibonectin levels in patients developing ARDS. Am Rev Respir Dis 127:95 (abstr)Google Scholar
  23. 23.
    McCullough B, Schneider S, Greene ND, Johanson WG, Jr (1978) Bleomycin-induced lung injury in baboons: alteration of cells and immunoglobins recoverable by bronchoalveolar lavage. Lung 155:337–358PubMedGoogle Scholar
  24. 24.
    McGuire WM, Spragg RG, Cohen AB, Cochrane CG (1982) Studies on the pathogenesis of the adult respiratory distress syndrome. J Clin Invest 69:543–553PubMedGoogle Scholar
  25. 25.
    Miles LA, Plow EF, Donnelly KJ, Hougie C, Griffin JH (1982) A bleeding disorder due to deficiency ofα 2-antiplasmin. Blood 59:1246–1251PubMedGoogle Scholar
  26. 26.
    Montgomery AB, Stager MA, Carrico CJ, Hudson LD (1985) Causes of mortality in patients with the adult respiratory distress syndrome. Am Rev Respir Dis 132:485–489PubMedGoogle Scholar
  27. 27.
    Nerlich AG, Nerlich ML, Joachim H, Muller PK (1984) Biochemical changes in parenchymal lung tissue associated with posttraumatic respiratory failure. Eur J Clin Invest 14:190–198PubMedCrossRefGoogle Scholar
  28. 28.
    Newman RA, Kimberly PJ, Stewart JA, Kelley J (1980) Assessment of bleomycin lung toxicity using angiotensin-converting enzyme in pulmonary lavage. Cancer Res 40:3621–3626PubMedGoogle Scholar
  29. 29.
    Petty TL, Ashbaugh DG (1971) The adult respiratory distress syndrome: clinical features and factors influencing prognosis and principles of management. Chest 70:233–239Google Scholar
  30. 30.
    Shaw JO, Ferrigni KS, Wetsel RA, Andrews CP, Collins JF, Fowler SR, Kolb WP (1986) Enzymes capable of cleaving the fifth component of complement in bronchoalveolar lavage fluid from patients with adult respiratory distress syndrome: Generation of chemotactic activity in airspaces. Submitted.Google Scholar
  31. 31.
    Thoman PJ, Shaw SL, Collins JF (1985) Isolation and characterization of fibronectin from baboon plasma. Connective Tissue Res 13:135–143Google Scholar
  32. 32.
    Woollen JW, Heyworth R, Walker PG (1961) Studies on glucosaminidase. 3. Testicular N-acetyl-beta-galactosaminidase. Biochem J 78:111–116PubMedGoogle Scholar
  33. 33.
    Zapol WM, Trelstad RL, Coffey JW, Tsai I, Salvador RA (1979) Pulmonary fibrosis in acute respiratory failure. Am Rev Respir Dis 119:457–454Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • James F. Collins
    • 1
    • 2
  • Ruben de los Santos
    • 1
    • 2
  • W. G. Johanson
    • 1
    • 2
  1. 1.Departments of Medicine and BiochemistryThe University of Texas Health Science Center at San Antonio, the Southwest Foundation for Biomedical ResearchUSA
  2. 2.Pulmonary Disease SectionThe Audie L. Murphy VA HospitalSan AntonioUSA

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