Journal of Biosciences

, Volume 29, Issue 4, pp 423–429 | Cite as

Maxillary palp glomeruli and ipsilateral projections in the antennal lobe ofDrosophila melanogaster



The antennal lobe was examined by Golgi-silver impregnation to differentiate the glomeruli depending on the source and types of inputs. Thirty-five of the 43 ‘identified’ olfactory glomeruli were Golgi-silver impregnated in the present study. Seven glomeruli compared to three, reported previously, were found to be targets of maxillary palp chemosensory neurons. These include glomeruli VA3, VC2, VM5, VA7m/VA7l of the ventral antennal lobe and DC2, DC3, DM5 of the dorsal antennal lobe. The number of glomeruli receiving the maxillary palp sensory projections tallies with the number ofDrosophila olfactory receptors (seven) reported to be expressed exclusively in the maxillary palp. Twenty-eight Golgi-impregnated glomeruli were found to receive input from the antennal nerve. The ratio of glomeruli serving the maxillary palp to those serving the antenna (∼1:5) matches with the ratio ofDrosophila olfactory receptors expressed in these two olfactory organs respectively. In addition to glomerulus V, glomeruli VP1-3, VL1, VL2a/2p and VC3m/3l were found to receive ipsilateral projections. Thus, additional ipsilateral glomeruli have been identified.


Antennal lobe Drosophila glomeruli olfaction 

Abbreviations used


Antennal lobe


Drosophila olfactory receptor


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  1. Borst A and Heisenberg M 1982 Osmotropotaxis inDrosophila melanogaster;J. Comp. Physiol. A 147 479–484CrossRefGoogle Scholar
  2. Christensen T A and Hildebrand J G 1987 Functions, organization and physiology of the olfactory pathways in the lepidopteran brain; inArthropod brain: its evolution, development, structure and function (ed.) A P Gupta (New York: John Wiley) pp 457–484Google Scholar
  3. Clyne P J, Warr C G, Freeman M R, Lessing D, Kim J and Carlson J R 1999 A novel family of divergent seven-trans-membrane proteins: candidate odorant receptors inDrosophila;Neuron 22 327–338PubMedCrossRefGoogle Scholar
  4. Devaud J-M 2003 Experimental studies of adultDrosophila chemosensory behaviour;Behavioural Processes 64 177–196PubMedCrossRefGoogle Scholar
  5. de Bruyne M, Clyne P J and Carlson J R 1999 Odour coding in a model olfactory organ: TheDrosophila maxillary palp;J. Neurosci. 19 4520–4532PubMedGoogle Scholar
  6. Fiala A, Spall T, Diegelmann S, Eisermann B, Sachse S, Devaud J M, Buchner E and Galizia L G 2002 Genetically expressed Cameleon inDrosophila melanogaster is used to visualize olfactory information in projection neurons;Curr. Biol. 12 1877–1884PubMedCrossRefGoogle Scholar
  7. Foelix R F, Stocker R F and Steinbrecht R A 1989 Fine structure of a sensory organ in the arista ofDrosophila melanogaster and some other Dipterans;Cell. Tissue Res. 258 277–287PubMedCrossRefGoogle Scholar
  8. Galizia C G, McIlwrath S L and Menzel R 1999 A digital three-dimensional atlas of the honeybee antennal lobe based on optical sections acquired by confocal microscopy;Cell Tissue Res. 295 383–394PubMedCrossRefGoogle Scholar
  9. Gao Q and Chess A 1999 Identification of candidateDrosophila olfactory receptors from genomic DNA sequence;Genomics 60 31–39PubMedCrossRefGoogle Scholar
  10. Gao Q, Yuan B and Chess A 2000 Convergent projections ofDrosophila olfactory neurons to specific glomeruli in the antennal lobe;Nature Neurosci. 3 780–785PubMedCrossRefGoogle Scholar
  11. Hildebrand J G and Shepherd G M 1997 Mechanism of olfactory discrimination: converging evidence for common principles across phyla;Annu. Rev. Neurosci. 20 595–631PubMedCrossRefGoogle Scholar
  12. Keller A and Vosshall L B 2003 Decoding olfaction inDrosophila;Curr. Opinion Neurobiol. 13 103–110CrossRefGoogle Scholar
  13. Laissue P P, Reiter C, Hiesinger P R, Halter S, Fischbach K F and Stocker R F 1999 Three-dimensional reconstruction of the antennal lobe inDrosophila melanogaster;J. Comp. Neurol. 405 543–552PubMedCrossRefGoogle Scholar
  14. Ng M, Roorda R D, Lima Q L, Zemelman B V, Morcillo P and Mesenbock G 2002 Transmission of olfactory information between populations of nerurons in the antennal lobe of the fly;Neuron 36 463–474PubMedCrossRefGoogle Scholar
  15. Shanbhag S R, Singh K and Singh R N 1995 Fine structure and primary sensory projections of sensilla located in the sacculus of the antenna ofDrosophila melanogaster;Cell Tissue Res. 282 237–249PubMedGoogle Scholar
  16. Shanbhag S R, Muller B and Steinbrecht R A 1999 Atlas of olfactory organs ofDrosophila melanogaster 1. Types, external organization, innervation and distribution of olfactory sensilla;Int. J. Insect Morphol. Embryol. 28 377–397CrossRefGoogle Scholar
  17. Shanbhag S R, Muller B and Steinbrecht R A 2000 Atlas of olfactory organ ofDrosophila melanogaster 2. Internal organization and cellular architecture of olfactory sensilla;Arthropod Struct. Dev. 29 211–229CrossRefGoogle Scholar
  18. Singh R N and Nayak S V 1985 Fine structure and primary sensory projections of sensilla on the maxillary palp ofDrosophila melanogaster meigen (Diptera: Drosophilidae);Int. J. Insect. Morphol. Embryol. 14 291–306CrossRefGoogle Scholar
  19. Stocker R F 2001Drosophila as a focus in olfactory research: Mapping of olfactory sensilla by fine structure, odor specificity, odorant receptor expression and central connectivity;Microsc. Res. Tech. 55 284–296PubMedCrossRefGoogle Scholar
  20. Stocker R F, Singh R N, Schorderet M and Siddiqi O 1983 Projection patterns of different types of antennal sensilla in the antennal glomeruli ofDrosophila melanogaster;Cell Tissue Res. 232 237–248PubMedCrossRefGoogle Scholar
  21. Stocker R F, Lienhard M C, Borst A and Fischbach K F 1990 Neuronal architecture of the antennal lobe inDrosophila melanogaster;Cell Tissue Res. 262 9–34PubMedCrossRefGoogle Scholar
  22. Vosshall L B, Wong A M and Axel R 2000 An olfactory sensory map in the fly brain;Cell 102 147–159PubMedCrossRefGoogle Scholar
  23. Wang J W, Wong A M, Flores J, Vosshall L B and Axel R 2003 Two-photon calcium imaging reveals an odor evoked map of activity in the fly brain;Cell 112 271–282PubMedCrossRefGoogle Scholar
  24. Wilson R I, Turner G C and Laurent G 2004 Transformation of olfactory representations in theDrosophila antennal lobe;Science 303 366–370PubMedCrossRefGoogle Scholar

Copyright information

© Indian Academy of Sciences 2004

Authors and Affiliations

  1. 1.Department of Applied ZoologyMangalore UniversityMangaloreIndia

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