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Journal of Biosciences

, Volume 23, Issue 5, pp 605–612 | Cite as

Fatty acid binding proteins from developing human fetal brain: Characterization and binding properties

  • Durba Mukhopadhyay
  • Prabar K. Ghosh
  • Aparna Sen
  • Manju Mukherjea
Article
  • 38 Downloads

Abstract

Two fatty acid binding proteins (FABPs) of identicalM r, 13 kDa, have been isolated from developing human fetal brain. A delipidated 105,000 g supernatant was incubated with [1 -14C]oleate and subjected to a Sephacryl S-200 column followed by gel filtration chromatography on a Sephadex G-75 column and ion-exchange chromatography using a DEAE-Sephacel column. Purity was checked by UV spectroscopy, SDS-PAGE, isoelectric focusing and immunological cross-reactivity. The two FABPs designated as DE-I (pI 5.4) and DE-II (pI 6.9) showed cross-reactivity with each other and no alteration at the antigenic site during intrauterine development. Anti-human fetal brain FABP does not cross-react with purified human fetal heart, gut, lung or liver FABPs. The molecular mass of DE-I and DE-II is lower than those of fetal lung and liver FABPs. Like liver FABP, these proteins bind organic anions, fatty acids and acyl CoAs but differ in their binding affinities. Both DE-I and DE-II have been found to exhibit higher affinity for oleate (K d = 0.23 μM) than palmitate (K d = 0.9μM) or palmitoyl-CoA (K d = 0.96 μM), with DE-I binding less fatty acids than DE-II. DE-II is more efficient in transferring fatty acid from phospholipid vesjcles than DE-I indicating that human fetal brain FABPs may play a significant role in fatty acid transport in developing fetal brain.

Keywords

Fatty acid binding protein fetal brain liposome 

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Copyright information

© Indian Academy of Sciences 1998

Authors and Affiliations

  • Durba Mukhopadhyay
    • 1
  • Prabar K. Ghosh
    • 1
  • Aparna Sen
    • 1
  • Manju Mukherjea
    • 1
  1. 1.Department of Biochemistry, University College of ScienceUniversity of CalcuttaCalcuttaIndia

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