Advertisement

Korean Journal of Chemical Engineering

, Volume 22, Issue 4, pp 552–555 | Cite as

Mixing and enzyme reactions in a microchannel packed with glass beads

  • Ken-Ichiro Sotowa
  • Ryo Miyoshi
  • Chan-Gi Lee
  • Yong Kang
  • Katsuki Kusakabe
Article

Abstract

Mixing behavior in a microchannel was investigated by means of a spectrophotometer equipped with optical fiber probes. Microreactors with a Y-shaped channel were fabricated on poly(methyl methacrylate) substrates. Glass beads were packed in the intersection and the downstream regions to improve mixing performance. An NaOH solution and a BTB solution as a pH indicator were fed to the microchannel with a syringe pump. As the mixing progressed, the color of the mixed solution changed to blue. The degree of mixing was evaluated by the change in absorbance at 623 nm of the mixing solution. The packed glass beads strongly enhanced the mixing performance in the microchannels, especially in the case of packing in the intersection. The effect of mixing in the microchannel on an enzyme reaction was also investigated. The hydrolysis of o-nitrophenyl-\-D-galactopyranoside by \-galactosidase was used as a model reaction. The results showed that the enzyme reaction was enhanced in the microreactor with glass beads compared with a batch reactor. The microreactor packed with glass beads gave the highest reaction rate.

Key words

Microchannel Mixing Enzyme Reaction Glass Beads o-Nitrophenyl-\-D-galactopyranoside 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bertsch, A., Heimgartner, S., Cousseau, P. and Renaud, P.,“Static Micromixers Based on Large Scale Industrial Mixer Geometry,”Lab-on-a-Chip, 1, 56 (2001).CrossRefGoogle Scholar
  2. Ehrfeld, W., Hessel, V. and Löwe, H.,Microreactors, Wiley-VCH, Weinheim, Germany, p. 41–85 (2000).CrossRefGoogle Scholar
  3. Hessel, V. and Löwe, H.,“Microchemical Engineering: Components, Plant Concepts, User Acceptance Part III,”Chem. Eng. Technol., 26, 531(2003).CrossRefGoogle Scholar
  4. Hinsmann, P., Frank, J., Svasek, P., Harasek, M. and Lendl B.,“Design, Simulation and Application of a New Micromixing Device for Time Resolved Infrared Spectroscopy of Chemical Reactions in Solution,”Lab-on-a-Chip, 1, 16 (2001).CrossRefGoogle Scholar
  5. Kanno, K.-I., Maeda, H., Izumo, S., Takeshita, K., Tashiro, A. and Fujii, M.,“Rapid Enzymatic Transglycosylation and Oligosaccharide Synthesis in a Microchip Reactor,”Lab on a Chip, 2, 15 (2002).CrossRefGoogle Scholar
  6. Kawazumi, H., Tashiro, A., Ogino, K. and Maeda, H.,“Observation of Fluidic Behavior in a Polymethylmethacrylate-fabricated by a Simple Spectroscopic Analysis,”Lab on a Chip,2, 8 (2002).CrossRefGoogle Scholar
  7. Losey, M. W., Schmidt, M. A. and Jensen, K. F.,“Microfabricated Multiphase Packed-bed Reactors: Characterization of Mass Transfer and Reactions,”Ind. Eng. Chem. Res., 40, 2555 (2001).CrossRefGoogle Scholar
  8. Miyazaki, M., Nakamura, H. and Maeda, H.,“Improved Yield of Enzyme Reaction in Microchannel Reactor,”Chem. Lett., 442 (2001).Google Scholar
  9. Sato, K., Tokeshi, M., Kimura, H. and Kitamori, T.,“Determination of Carcioembryonic Antigen in Human Sera by Integrated Bead Bed Immunoassay in a Microchip for Cancer Diagnosis,”Anal. Chem., 73, 1213(2001).CrossRefGoogle Scholar
  10. Schwesinger, N., Frank, T. and Wurmus, H.,“A Modular Microfluid System with an Integrated Micromixer,”J. Micromech. Microeng., 6, 99(1996).CrossRefGoogle Scholar
  11. Seong, G. H. and Crooks, R. M.,“Efficient Mixing and Reactions within Microfluidic Channels using Microbead-supported Catalysis,”JACS, 124, 13360 (2002).CrossRefGoogle Scholar
  12. Tanaka, Y., Slydnev, M. N., Hibara, A., Tokeshi, M. and Kitamori, T.,“Non-contact Photothermal Control of Enzyme Reactions on a Microchip by using a Compact Diode Laser,”J. Chromatogr. A, 894, 45 (2000).CrossRefGoogle Scholar
  13. Yamaguchi, Y., Takagi, F., Yamashita, K., Maeda, H., Sotowa, K. I., Kusakabe, K., Yamasaki, Y. and Morooka, S.,“3-D Simulation and Visualization of Laminar Flow in a Microchannel with Hair-pin Curves,”AIChEJ., 50, 1530(2004).CrossRefGoogle Scholar

Copyright information

© Korean Institute of Chemical Engineering 2005

Authors and Affiliations

  • Ken-Ichiro Sotowa
    • 1
  • Ryo Miyoshi
    • 1
    • 2
  • Chan-Gi Lee
    • 1
    • 2
  • Yong Kang
    • 1
    • 3
  • Katsuki Kusakabe
    • 1
    • 3
  1. 1.Department of Chemical Science and TechnologyThe University of TokushimaTokushimaJapan
  2. 2.Department of Applied ChemistryKyushu UniversityFukuokaJapan
  3. 3.Department of Chemical EngineeringChungnam National UniversityDaejeonKorea

Personalised recommendations