Cadmium-induced microsomal membrane-bound peroxidases mediated hydrogen peroxide production in barley roots
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The effect of cadmium on microsomal membrane-bound peroxidases and their involvement in hydrogen peroxide production was studied in barley roots. One anionic and two cationic peroxidases were detected, which were strongly activated by Cd treatment. Positive correlation was found between root growth inhibition and increased peroxidase, NADH oxidase activity and H2O2 generation in root microsomal membrane fraction of Cd-treated barley roots.
Key wordsoxidative stress peroxidase isozymes root growth inhibition
List of abbreviations
reactive oxygen species
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- Chance B., Maehly A.C. 1995. Assay of catalases and peroxidases. In: Colowick SP, Kaplan NO (eds) Methods in Enzymology, Vol 2. Academic Press, New York, NY, pp 764–775.Google Scholar
- Chen S.L., Kao C.H. 1995. Cd induced changes in proline level and peroxidase activity in roots of rice seedlings. Plant growth regul., 17: 67–71.Google Scholar
- Ishida A., Ookubo K., Ono K. 1987. Formation of hydrogen peroxide by NAD(P)H oxidation with isolated cell wall-associated peroxidase from cultured liverwort cells, Marchantia polymorpha L. Plant Cell Physiol., 28: 723–726.Google Scholar
- Metwally A., Safranova V.I., Belimov A.A., Dietz K. 2005. Genotypic variation of the response to cadmium toxicity in Pisum sativum L. J. Exp. Bot., 56: 167–178.Google Scholar
- Šimonovičová M., Bočová B., Huttová J., Mitrík I., Tamás L. 2005. Effect of cadmium on oxalate oxidase activity in barley roots. Biologia, 60: 463–466.Google Scholar