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Journal of Biosciences

, Volume 1, Issue 2, pp 125–132 | Cite as

Interaction of allylisothiocyanate with bovine serum albumin

  • N. V. Kishore Kumar Murthy
  • M. S. Narasinga Rao
Article

Abstract

The interaction of allylisothiocyanate with bovine serum albumin was monitored by fluorescence titration. The interaction was weak with an apparent association constant of 2 × 102. The interaction was unaffected in the pH range of 5.0 to 8.3 and by NaCl. However, the addition of dioxane upto 4% increased the value of the association constant. N-Methyl bovine serum albumin and bovine serum albumin with sulphydryl groups blocked had the same affinity for allylisothiocyanate suggesting that amino and sulphydryl groups may not be involved in the interaction. Polyacrylamide gel electrophoresis and estimation of available lysine suggested that there were perhaps two types of groups involved in the interaction of allylisothiocyanate with bovine serum albumin.

Keywords

Serum albumin allylisothiocyanate interaction 

Abbreviations used

AIT

allylisothiocyanate

BSA

bovine serum albumin

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Copyright information

© Indian Academy of Sciences 1979

Authors and Affiliations

  • N. V. Kishore Kumar Murthy
    • 1
  • M. S. Narasinga Rao
    • 1
  1. 1.Central Food Technological Research InstituteMysore

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