Mathematical modeling for the estimation of lipase activity by agar diffusion method
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A mathematical model was developed to estimate the lipase activities from the experimentally obtained physical dimension of halo, formed by a diffusing lipase through agar media. A diffusion equation based upon the mass balance was derived in cylindrical coordinates, the integration of which was carried out by using finite difference method. A regressional analysis of the experimental data using the proposed model has brought the values for the hindered diffusivity of lipase through agar media and the minimum effective lipase concentration of 0.817 × 10−6 cm2 /s and 2.86 unit/ cm3, respectively. The procedures, delineated in this work are considered to be useful for the quantitative analysis of haloforming enzymes at predetermined standard conditions.
Key wordsLipase Activity Halo Agar Modeling
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- Constantinides, A.,“Applied Numerical Methods with Personal Computers”, McGraw-Hill, New York, 1987.Google Scholar
- Fransmana, M., Junne, G. and Roobeek, A.,“The Biotechnology Revolution?”, Blackwell, Oxford, 1995.Google Scholar
- IMSL,“IMSL.LIBRARY User’s Manual, Vol. 4”, IMSL, Houston, 1984.Google Scholar
- Kouker, G. and Jaeger, K.-E.,“Specific and Sensitive Plate Assay for Bacterial Lipases”,Appl. and Environm. Microbiol.,53(1), 211 (1987).Google Scholar
- Lee, S.-Y. and Rhee, J.-S.,“Detection and Determination of Lipase Activity”,J. of Mcirobiol. and Biotech.,4(2), 85 (1994).Google Scholar