Advertisement

Korean Journal of Chemical Engineering

, Volume 13, Issue 3, pp 282–287 | Cite as

Diffusivity of bacteria

  • Yeong-Chul Kim
Article

Abstract

The effects of motility and aggregation on the diffusion coefficient for bacteria were studied in an aqueous system. The effects of cell concentrations, capillary tube sizes, and dilution rates on the diffusion coefficient were examined. In general, motile cells can diffuse about 1000 times faster than non-motile cells.Pseudomonas aeruginosa, a motile cell, andKlebsiella pneumoniae, a non-motile cell, were used for this research. Diffusion coefficients were measured by the capillary tube assay developed by Adler [1969]. From this procedure the diffusion coefficient ofPseudomonas aeruginosa was 2.1×10−5 (standard deviation: 1.0× 10−5) cm2/s and that ofKlebsiella pneumoniae was 0.9×10−5 (standard deviation : 0.5 × 10−5) cm2/s. The diffusion coefficient ofPseudomonas aeruginosa was about 2.3 times higher than that ofKlebsiella pneumoniae. The Stokes-Einstein equation could not be used for estimating the diffusion coefficients forKlebsiella pneumoniae andPseudomonas aeruginosa. The experimental value for the diffusion coefficient ofKlebsiella pneumoniae was about 2000 times higher than that (4.5×10−9 cm2/s) obtained from the Stokes-Einstein equation. This discrepancy was due to the aggregation ofKlebsiella pneumoniae.

Key words

Diffusivity Bacteria Aggregation Motile Non Motile 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Adler, J., “Chemoreceptors in Bacteria”,Science,166, 1588 (1969).CrossRefGoogle Scholar
  2. Adler, J.,“A Method for Measuring Chemotaxis and Use of the Method to Determine Optimum Conditions for Chemotaxis byEscherichia coif, J. of General Microbiology,74, 77 (1973).Google Scholar
  3. Adler, J. and Margaret, M. D., “A Method for Measuring the Motility of Bacteria and for Comparing Random and Nonrandom Motility”,J. of General Microbiology,46, 161 (1967).Google Scholar
  4. Adler, J. and Bonnie Templeton,“The Effect of Environmental Conditions on the Motility ofEscherichia coli”,J. of General Microbiology,46, 175 (1967).Google Scholar
  5. Berg, H. and Brown, D., Chemotaxis inEscherichia coli Analyzed by Three-Dimensional Tracking”,Nature,239, 500 (1972).CrossRefGoogle Scholar
  6. Bergey’s Manual of Determinative Bacteriology, 8th ed., Williams & Wilkins Company (1974).Google Scholar
  7. Dahlquist, F. W., Lovely, P. and Koshland, D. E.,“Quantitative Analysis of Bacterial Migration in Chemotaxis”,Nature New Biology,236, 120 (1972).CrossRefGoogle Scholar
  8. Fahien, R.,“Fundamentals of Transport Phenomena”, McGraw-Hill Book Company (1983).Google Scholar
  9. Lovely, P. S. and Dahlquist, F. W.,“Statistical Measures of Bacterial Motility and Chemotaxis”,J. of Theoretical Biology,50, 477 (1975).CrossRefGoogle Scholar
  10. Nossal, R. and Chen, S.,“Light Scattering from Motile Bacteria”,Journal de Physique,33(C1), 171 (1972).Google Scholar
  11. Nossal, R. and Weiss, G.,“Analysis of a Densitometry Assay for Bacterial Chemotaxis”,J. Theor. Biol.,41, 143(1973).CrossRefGoogle Scholar
  12. Schneider, W. R. and Doetsch, R. N.,“Effect of Viscosity on Bacterial Motility”,J. of Bacteriology,117, 696 (1974).Google Scholar
  13. Segel, L. A., Ilan Chet, and Yigal Henis,“A Simple Quantitative Assay for Bacterial Motility”,J. of General Microbiology,98, 329 (1977).Google Scholar
  14. Shoesmith, J. G., “The Measurement of Bacterial Motility”,J. of General Microbiology,22, 528 (1960).Google Scholar
  15. Slater, N., Powell, M. and Johnson, P., The Relevance of Bacterial Motility to Fermenter Contamination: An Experimental Study forBacillus cereus”,Trans I ChemE,59, 170 (1981).Google Scholar
  16. Stock, G.,“The Measurement of Bacterial Translation by Photon Correlation Spectroscopy”,Biophys.,22, 79 (1978).CrossRefGoogle Scholar
  17. Thonemann, P. C. and Evans, C. J., “The Dispersal of an Initial Concentration of Motile Bacteria”,J. of General Microbiology,92, 25 (1976).Google Scholar
  18. Vaituzis, Z. and Doetsch, R. N.,“Motility Tracks: Technique for Quantitative Study of Bacterial Movement ”,Applied Microbiology,17, 584(1969).Google Scholar

Copyright information

© Korean Institute of Chemical Engineering 1996

Authors and Affiliations

  • Yeong-Chul Kim
    • 1
  1. 1.Cheiljedang Corporation, R & D CenterBioprocess Research TeamKyonggi-DoKorea

Personalised recommendations