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In situ berberine separation with immobilized adsorbent in cell suspension cultures ofThalictrum rugosum

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Abstract

The use of suspended and alginate-entrapped XAD-7, polycarboxyl ester resin, for thein situ separation of berberine, isoquinoline alkaloid, produced from plant cell culture ofThalictrum rugosum was investigated. XAD-7 could adsorb the berberine and the amount of berberine adsorbed on XAD-7 depended on pH. The neutral form of berberine was adsorbed onto XAD-7 and the adsorption isotherm for berberine showed a Langmuir-type appearance.In situ berberine removal enhanced the production of secondary metabolites in cell suspension culture ofThalictrum rugosum. Addition of XAD-7 at the exponential phase of cell growth was the most effective for enhancement of berberine production. In chitosan-treated cell culture to permeabilize intracellular berberine, berberine secretion was significantly accelerated by addition of alginate-entrapped XAD-7 at the stationary phase of cell growth and thus more than 70% of the produced berberine could be adsorbed to alginate-entrapped XAD-7.

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Abbreviations

As :

amount of the adsorbent [g]

C:

berberine concentration [mg/mL]

C a o :

adsorbed berberine concentration [mg/mL]

Co :

initial berberine concentration [mg/mL]

C+ :

concentration of protonated forms of berberine [mg/mL]

Co :

concentration of unprotonated forms of berberine [mg/mL]

Q:

resin loading capacity [mg adsorbed/g adsorbent]

VB :

liquid volume of berberine solution [mL]

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Authors and Affiliations

  1. Department of Chemical Engineering, Sogang University, C.P.O. Box 1142, Seoul, Korea

    Jeong-Woo Choi

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  1. Jeong-Woo Choi
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Choi, JW. In situ berberine separation with immobilized adsorbent in cell suspension cultures ofThalictrum rugosum . Korean J. Chem. Eng. 9, 128–134 (1992). https://doi.org/10.1007/BF02705129

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  • DOI: https://doi.org/10.1007/BF02705129

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