Korean Journal of Chemical Engineering

, Volume 9, Issue 3, pp 128–134 | Cite as

In situ berberine separation with immobilized adsorbent in cell suspension cultures ofThalictrum rugosum

  • Jeong-Woo Choi


The use of suspended and alginate-entrapped XAD-7, polycarboxyl ester resin, for thein situ separation of berberine, isoquinoline alkaloid, produced from plant cell culture ofThalictrum rugosum was investigated. XAD-7 could adsorb the berberine and the amount of berberine adsorbed on XAD-7 depended on pH. The neutral form of berberine was adsorbed onto XAD-7 and the adsorption isotherm for berberine showed a Langmuir-type appearance.In situ berberine removal enhanced the production of secondary metabolites in cell suspension culture ofThalictrum rugosum. Addition of XAD-7 at the exponential phase of cell growth was the most effective for enhancement of berberine production. In chitosan-treated cell culture to permeabilize intracellular berberine, berberine secretion was significantly accelerated by addition of alginate-entrapped XAD-7 at the stationary phase of cell growth and thus more than 70% of the produced berberine could be adsorbed to alginate-entrapped XAD-7.


Chitosan Cell Suspension Culture Berberine Plant Cell Culture Shikonin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



amount of the adsorbent [g]


berberine concentration [mg/mL]


adsorbed berberine concentration [mg/mL]


initial berberine concentration [mg/mL]


concentration of protonated forms of berberine [mg/mL]


concentration of unprotonated forms of berberine [mg/mL]


resin loading capacity [mg adsorbed/g adsorbent]


liquid volume of berberine solution [mL]


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Copyright information

© Korean Institute of Chemical Engineering 1992

Authors and Affiliations

  • Jeong-Woo Choi
    • 1
  1. 1.Department of Chemical EngineeringSogang UniversitySeoulKorea

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