Journal of Biosciences

, Volume 11, Issue 1–4, pp 155–166 | Cite as

Generation of bacteriophage T3 mRNAs by post-transcriptional processing by RNase III

  • Hemanta K. Majumder
  • Samit ADhya
  • Umadas Maitra


The primary transcripts synthesizedin vitro from a T3 DNA template byEscherichia coli RNA polymerase and by T3 phage-specific RNA polymerase have been characterized with regard to cleavage by RNase III and the size of the products of the cleavage reaction have been compared with those ofin vivo T3 RNAs. It has been observed that the large RNA molecule synthesized invitro byEscherichia coli RNA polymerase from the early region of T3 DNA are cleaved at specific sites byEscherichia coli RNase III to produce all the early mRNAs normally observed in T3-infected cells. In contrast, evidence presented here shows that some of the late T3 mRNAs are generated as direct products of transcription of late regions of T3 DNA by T3 RNA polymerase without mediation of RNase III, while many other late T3 mRNAs are formed by RNase III cleavage of two of the high molecular weight T3 RNA polymerase transcripts. Thesein vitro data appear to be in good agreement with the observed sizes of late T3 mRNAs formedin vivo in T3-infected RNase III-deficient and RNaseIII + Escherichia coli cells.


RNA processing RNase III RNA nucleotidyl transferase T3 promoter 

Abbreviations used


Molecular weight


sodium dodecyl sulphate


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Adhya, S. (1981)Transcription of Bacteriophage T3 DNA Catalyzed by Escherichia coli and T3 RNA Poly-merases, Ph.D. Thesis, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York, USA.Google Scholar
  2. Adhya, S., Basu, S., Sarkar, P. and Maitra, U. (1981)Proc. Natl. Acad. Sci. USA,78, 147.PubMedCrossRefGoogle Scholar
  3. Adhya, S., Sarkar, P., Valenzuela, D. and Maitra, U. (1979)Proc. Natl. Acad. Sci. USA,76, 1613..PubMedCrossRefGoogle Scholar
  4. Bailey, J. N., Klement, J. F. and McAllister, W. T. (1983)Proc. Natl. Acad. Sci. USA,80, 2814.PubMedCrossRefGoogle Scholar
  5. Bailey, J. N. and McAllister, W. T. (1980)Nucleic Acids Res.,8, 5071.PubMedCrossRefGoogle Scholar
  6. Basu, S., Sarkar, P., Adhya, S. and Maitra, U. (1984)J. Biol. Chem.,259, 1993.PubMedGoogle Scholar
  7. Beier, H. and Hausmann, R. (1973)J. Virol.,12, 417.PubMedGoogle Scholar
  8. Beier, H., Golomb, M. and Chamberlin, M. (1977)J. Virol.,21, 753.PubMedGoogle Scholar
  9. Bishayee, S. and Maitra, U. (1976)Biochem. Biophys. Res. Commun.,73, 306.PubMedCrossRefGoogle Scholar
  10. Chakraborty, P. R., Bandyopadhyay, P., Huang, H. H. and Maitra, U. (1974)J. Biol. Chem.,249, 6901.PubMedGoogle Scholar
  11. Chakraborty, P. R., Salvo, R. A., Majumder, H. and Maitra, U. (1977)J. Biol. Chem.,252, 6485.PubMedGoogle Scholar
  12. Chakraborty, P. R., Sarkar, P., Huang, H. H. and Maitra, U. (1973)J. Biol. Chem.,248, 6637.PubMedGoogle Scholar
  13. Chamberlin, M., McGrath, J. and Waskell, L. (1970)Nature (London),228, 227.CrossRefGoogle Scholar
  14. Chamberlin, M. and Ryan, T. (1982)Enzymes,15, 87.Google Scholar
  15. Dunn, J. J. (1976)J. Biol. Chem.,251, 3807.PubMedGoogle Scholar
  16. Dunn, J. J., Bautz, F. A. and Bautz, E. K. F. (1971)Nature New Biol.,230, 94.PubMedGoogle Scholar
  17. Dunn, J. J., McAllister, W. T. and Bautz, E. K. F. (1972)Proc. Natl Acad. Sci. USA,70, 1559.CrossRefGoogle Scholar
  18. Dunn, J. J. and Studier, F. W. (1973)Proc. Natl. Acad. Sci. USA,70, 3296.PubMedCrossRefGoogle Scholar
  19. Dunn, J. J. and Studier, F. W. (1975)J. Mol. Biol.,99, 487.PubMedCrossRefGoogle Scholar
  20. Floyd, R. W., Stone, M. P. and Joklik, W. K. (1974)Anal. Biochem.,59, 599.PubMedCrossRefGoogle Scholar
  21. Franklin, R. M. (1966)Proc. Natl. Acad. Sci. USA,55, 1504.PubMedCrossRefGoogle Scholar
  22. Hausmann, R. (1973)Annu. Rev. Microbiol.,27, 51.CrossRefGoogle Scholar
  23. Golomb, M. and Chamberlin, M. (1977)J. Virol.,21, 743.PubMedGoogle Scholar
  24. Issinger, O. G. and Hausmann, R. (1973)J. Virol,11, 465.PubMedGoogle Scholar
  25. Koller, T., Sago, J. M. and Bujard, H. (1974)Biopolymers,13, 995.PubMedCrossRefGoogle Scholar
  26. Landy, A., Abelson, J., Goodman, H. M. and Smith, J. D. (1976)J. Mol. Biol.,29, 457.CrossRefGoogle Scholar
  27. Maitra, U.(1971)Biochem. Biophys. Res. Commun.,43, 443.PubMedCrossRefGoogle Scholar
  28. Maitra, U. and Hurwitz, J. (1967)J. Biol. Chem.,242, 4897.PubMedGoogle Scholar
  29. Majumder, H. K., Bishayee, S., Chakraborty, P. R. and Maitra, U. (1977)Proc. Natl. Acad. Sci. USA,74, 4891.PubMedCrossRefGoogle Scholar
  30. Majumder, H. K., Maitra, U. and Rosenberg, M. (1979)Proc. Natl. Acad. Sci. USA,74, 4891.CrossRefGoogle Scholar
  31. Neff, N. F. and Chamberlin, M. (1978)J. Biol. Chem.,253, 2455.PubMedGoogle Scholar
  32. Robertson, H. D. and Dunn, J. J. (1975)J. Biol. Chem.,250, 3050.PubMedGoogle Scholar
  33. Robertson, H. D. and Hunter, T. (1975)J. Biol. Chem.,250, 418.PubMedGoogle Scholar
  34. Rosenberg, M., Kramer, R. A. and Steitz, J. A. (1974)J. Mol. Biol.,89, 772.CrossRefGoogle Scholar
  35. Sarkar, P., SenGupta, D., Basu, S. and Maitra, U. (1985)Gene,33, 351.PubMedCrossRefGoogle Scholar
  36. Studier, F. W. and Movva, N. (1976)J. Virol,19, 136.PubMedGoogle Scholar
  37. Summers, W. C. (1970)Anal. Biochem.,33, 449.CrossRefGoogle Scholar
  38. Summers, W. C. (1972)Annu. Rev. Genet.,6, 191.PubMedCrossRefGoogle Scholar
  39. Summers, W. C. and Siegel, R. B. (1970)Nature (London),228, 1160.CrossRefGoogle Scholar

Copyright information

© Indian Academy of Sciences 1987

Authors and Affiliations

  • Hemanta K. Majumder
    • 1
  • Samit ADhya
    • 1
  • Umadas Maitra
    • 1
  1. 1.Department of Developmental Biology and Cancer, Division of BiologyAlbert Einstein College of MedicineBronxUSA

Personalised recommendations